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Preparation method of engineered vaccine based on CSF-FMD duplex gene

A technology of CSF-FMD and genetic engineering vaccines, which is applied in the field of preparation of CSF-FMD dual genetic engineering vaccines, can solve problems such as unavoidable safety issues, and achieve the goal of reducing economic losses, good immune effect, and strong specificity Effect

Inactive Publication Date: 2014-07-23
GUIZHOU UNIV
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Problems solved by technology

However, both attenuated vaccines and inactivated vaccines are vaccines made of intact virus particles, and neither can escape safety issues

Method used

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  • Preparation method of engineered vaccine based on CSF-FMD duplex gene

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Embodiment Construction

[0015] Embodiments of the invention:

[0016] The main raw materials for the research and application of dual nucleic acid vaccine based on classical swine fever virus and porcine O-type foot-and-mouth disease virus:

[0017] Eukaryotic expression plasmid pcDNA3.1-CSFV-E2-FMDV-O-VP1, tryptone, yeast extract, sodium chloride, endotoxin-free plasmid extraction related reagents, etc.

[0018] The described research and application preparation steps based on classical swine fever virus and porcine O-type foot-and-mouth disease virus dual nucleic acid vaccine include:

[0019] First, the design and synthesis of primers

[0020] Login to GeneBank (www.ncbi.nlm.nih.gov) to obtain CSFV-GZ-2009 strain (accession number: HQ380231.1), C-ZJ-2008 strain (accession number: HM175885.1), JL1(06) strain ( Accession No.: EU497410.1) and Shimen / HVRI strain (Accession No.: AY775178.1) sequences were compared with Bioedit software and selected sequences were designed using Primer Premier 5 softw...

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Abstract

The invention relates to a preparation method of an engineered vaccine based on a CSF-FMD duplex gene, and discloses research and application of a duplex nucleic acid vaccine based on hog cholera virus-O type hog foot-and-mouth disease virus. Main materials of the duplex nucleic acid vaccine based on hog cholera virus-O type hog foot-and-mouth disease virus are as follows: pcDNA3.1-CSFV-E2-FMDV-O-VP1 eukaryotic plasmids. The preparation method disclosed by the invention researches nucleic acid vaccines for CSFVE2 gene and the FMDV-OVP1, can stimulate an animal body to generate corresponding high-level antibody and show very strong specificity. For the hog infection and propagation caused by CSFV and FMDV-O, the E2 gene and the VP1 gene achieve prevention and control effect on two viruses, so that safe and effective effects can be achieved.

Description

technical field [0001] The invention relates to the research and application of a CSF-FMD dual genetic engineering vaccine. Background technique [0002] Swine fever (Swine Fever, SF), also known as hog cholera (Hog Cholera, HC), also known as classical swine fever (Classical Swine Fever, CSF) in Europe, is caused by classical swine fever virus (Classical Swine Fever Virus, CSFV) A highly contagious, acute infectious disease. It is characterized by acute onset, high fever retention, degeneration of microcirculatory vessel wall and the resulting generalized small spot hemorrhage, multiple hemorrhage, necrosis and infarction in internal organs, and it is highly infectious and has a high fatality rate . It is listed as one of the legally reportable animal diseases by the World Organization of Animal Health (OIE), and it is listed as a first-class animal infectious disease in my country. The CSFV genome is a single-stranded positive-sense RNA with a total length of about 12.3...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K39/395A61P31/14A61K39/187A61K39/135
Inventor 王开功尹鑫周碧君文明程振涛王慧
Owner GUIZHOU UNIV
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