A method for inducing diapause of budworm
A technology of budworm and diapause, which is applied in animal husbandry and other fields, to achieve good effect, convenient operation and shorten the overall lifespan
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Embodiment 1
[0018] The newly hatched larvae of P. tabaci were raised in a 10cm-diameter petri dish with artificial feed until the third instar, and then raised in a 1.2cm×10cm glass test tube. The temperature was pre-controlled at 20°C, the light-to-dark ratio was 10:14, and the light intensity was 33%. . Then move the worms to an artificial climate box at 4°C with a relative humidity of 78% for 10 days, then place them in a refrigerator at 0°C for 15 days, repeat the treatment twice, and finally move the treated worms to 25°C Process 3d. Then cultivate normally, and then the diapause of P. tabaci can be made.
Embodiment 2
[0020] The newly hatched larvae of P. tabaci were raised in a 15m-diameter petri dish with artificial feed until the third instar, and then raised in a 1.2cm×10cm glass test tube. The temperature was pre-controlled at 25°C, the light-to-dark ratio was 10:14, and the light intensity was 31%. . Then move the worms to 4°C, 86% relative humidity in an artificial climate box for 10 days, then place them in a refrigerator at 0°C for 15 days, repeat the treatment 3 times, and finally move the treated worms to 32°C Process 3d. Then cultivate normally, and then the diapause of P. tabaci can be made.
Embodiment 3
[0022] The newly hatched larvae of P. tabacum were raised in a 13m-diameter petri dish with artificial feed until the third instar, and then raised in a 1.2cm×10cm glass test tube. The temperature was pre-controlled at 21°C, the light-to-dark ratio was 10:14, and the light intensity was 29%. . Then move the worms to 4°C, 80% relative humidity in an artificial climate box for 10 days, then put them in a refrigerator at 0°C for 15 days, repeat the treatment 3 times, and finally move the treated worms to 27°C Process 3d. Then cultivate normally, and then the diapause of P. tabaci can be made.
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