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A compound probiotic spray for preventing and treating neonatal livestock diarrhea and preparation method thereof

A technology of compound probiotics and sprays, which is applied in aerosol delivery, medical preparations containing active ingredients, drug combinations, etc., can solve the problem of lack of innate immunity, immature digestive function of young animals, and ineffectiveness of adult animals. To achieve the effect of maintaining normal physiological functions, simple and effective application methods, and improving intestinal micro-ecological environment

Active Publication Date: 2017-09-05
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Third, physiological factors, due to the lack of innate immunity and immature digestive function of young animals
Different types of preparations have different effects on animals at different physiological stages. For example, some preparations have good effects on young animals, but not on adult animals.
At present, we have searched Chinese patents and related literature on probiotic preparations, and found that most of them appear in the form of feed additives, and newborn young animals cannot eat feed, so the application of such probiotic preparations is limited in newborn young animals

Method used

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  • A compound probiotic spray for preventing and treating neonatal livestock diarrhea and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of the composite probiotic spray of the present embodiment is as follows:

[0021] 1) Inoculate Bacillus subtilis, Bacillus cereus, Enterococcus faecalis, and saccharomyces into their respective optimum medium, culture at 37°C for 24 hours, take 100ml of the culture solution and transfer it to a fermenter for expanded cultivation, when The concentration of viable bacteria in the fermentation broth reached 2×10 8 When CFU / ml is above, stop culturing. The fermentation broth was taken and centrifuged at 3000 rpm for 20 minutes to collect the bacteria.

[0022] 2) Evenly mix the above-mentioned bacteria in a weight ratio of 1:1:2.5:2, take 100 parts of the mixture, 300 parts of skimmed milk powder, 50 parts of mannose, and 50 parts of cysteine, and mix them uniformly at 50 rpm Stir at a high speed for 30 minutes, so that the cells are completely adsorbed on the carrier.

[0023] 3) Mix 400 parts of dextrin, 400 parts of sodium alginate and the mixt...

Embodiment 2

[0025] The preparation method of the composite probiotic spray of the present embodiment is as follows:

[0026] 1) Inoculate Bacillus licheniformis, Lactobacillus, Bifidobacterium, and Streptococcus thermophilus into their respective optimum culture medium, culture at 37°C for 36 hours, and transfer 100ml of the culture solution to a fermenter for expansion culture. The concentration of viable bacteria in the fermentation broth reached 2×10 8 When CFU / ml is above, stop culturing. The fermentation broth was taken and centrifuged at 4000 rpm for 15 minutes to collect the bacteria.

[0027] 2) Mix the above-mentioned bacteria uniformly in a weight ratio of 0.5:1.5:2:1, take 100 parts of the mixture, 200 parts of skimmed milk powder, 200 parts of soybean protein powder, and 50 parts of sodium lactate, and mix them uniformly at a speed of 50 rpm Stir for 30 minutes to make the bacteria completely adsorb on the carrier.

[0028] 3) Mix 600 parts of soluble starch, 200 parts of m...

Embodiment 3

[0030] The preparation method of the composite probiotic spray of the present embodiment is as follows:

[0031] 1) Inoculate Bacillus cereus, Bifidobacterium, Enterococcus faecalis, Lactobacillus plantarum, and Streptococcus thermophilus into their respective optimum culture medium, culture at 37°C for 24 hours, and transfer 100ml of the culture solution to the fermenter Expanded culture in the medium, when the concentration of viable bacteria in the fermentation broth reached 2×10 8 When CFU / ml is above, stop culturing. The fermentation broth was taken and centrifuged at 4500 rpm for 30 minutes to collect the bacteria.

[0032] 2) Mix the above-mentioned bacteria uniformly in a weight ratio of 1:2:2.5:1:1, take 200 parts of the mixture, 100 parts of skimmed milk powder, 300 parts of soybean protein powder, and 100 parts of cysteine, and mix them uniformly, and press 50 Stir at a speed of rpm for 30 minutes, so that the cells are completely adsorbed on the carrier.

[0033...

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Abstract

The invention discloses a composite probiotic spray for preventing and treating newborn livestock diarrhea, and discloses a preparation method of the spray. The composite probiotic spray for preventing and treating newborn livestock diarrhea is prepared from the following raw materials in parts by weight: 200-1,000 parts of adsorptive support, 400-2,000 parts of coated support and 100-200 parts of probiotic composition. The preparation has the effects of regulating intestinal micro-ecological balance of newborn young livestock, keeping normal physiological functions of intestines, enhancing immunity of organisms, improving the disease resistance of organisms, and in particular effectively preventing physiological and pathological diarrhea of young livestock. The composite probiotic spray is unique in dosage form, convenient and efficient to apply, more suitable for newborn young livestock, and is suitably popularized and applied in animal husbandry production.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicine, and in particular relates to a compound probiotic spray for preventing and treating neonatal livestock diarrhea and a preparation method thereof. Background technique [0002] Newborn livestock (including pigs, cattle, sheep, horses, etc.) diarrhea has always been one of the problems that plague the aquaculture industry, and it is also the main disease that endangers the aquaculture industry at present. According to statistics, in large-scale pig farms and sheep farms, the incidence of diarrheal disease in newborn piglets and lambs is as high as 75% and 62%. The growth performance of livestock is reduced, the feed conversion rate is reduced, the cost of breeding is increased, the benefit of breeding is reduced, and the economic loss caused by this can be billions of yuan every year. There are many reasons for neonatal diarrhea. First, pathogenic factors, including bacteria, viruses, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/064A61K35/742A61K35/747A61K35/745A61K35/744A61K9/12A61P1/12A61K35/741
Inventor 杨帆孙爱平秦卫玲魏纪东马玉龙
Owner XINXIANG MEDICAL UNIV
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