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Method for preparing bactericide from bran

A technology of agricultural fungicides and fungicides, which is applied in the directions of fungicides, botanical equipment and methods, and chemicals for biological control, etc., to achieve the effects of no pesticide residues and simple preparation process.

Inactive Publication Date: 2014-07-16
安徽省淮北市农药厂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, using bran as a raw material to select suitable strains for fermentation to prepare agricultural fungicides, which is suitable for large-scale production, has a safe and environmentally friendly production process, and has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Utilize bran to prepare the method for fungicide, its step is as follows:

[0014] 1. Ingredients: mix sugar: water = 1:10 until sugar is dissolved in water to form sugar water; Zygomyces bisporus: Bayer combined yeast: Acetobacter xylinum = 0:2.5:2.5 to form a mixed bacteria liquid;

[0015] 2. Fermentation: Mix the sugar water and the mixed bacterial solution in a ratio of 80:1 to form a mixed solution, put the bran into the fermentation tank layer by layer, and spray the mixed solution evenly after each layer until the layer is wet; keep the temperature at 26°C , fermented for 20 days;

[0016] 3. Preparation of bactericide: filter the fermented feed liquid to obtain fermented liquid and solid slag, add sodium hydroxide to the obtained fermented liquid to adjust the pH value to 6, and filter again to obtain flavonoid-containing apigenin, alkyl 5-nonadecyl resorcinol, 5-unacyl resorcinol, 5-tricosyl resorcinol of resorcinols, sugar pentosan, few A mixture of tettos...

Embodiment 2

[0018] Utilize bran to prepare the method for fungicide, its step is as follows:

[0019] 1. Ingredients: mix sugar: water = 1:20 until sugar is dissolved in water to form sugar water; Zygomyces bisporus: Bayer combined yeast: Acetobacter xylinum = 2:4:4 to form a mixed bacteria liquid;

[0020] 2. Fermentation: Mix the sugar water and the mixed bacterial solution in a ratio of 100:1 to form a mixed solution, put the bran layer by layer into the fermentation tank, and spray the mixed solution evenly after each layer until the layer is wet; keep the temperature at 30°C , fermented for 15 days;

[0021] 3. Preparation of bactericide: filter the fermented feed liquid to obtain fermented liquid and solid slag, add sodium hydroxide to the obtained fermented liquid to adjust the pH value to 8, and filter again to obtain flavonoid-containing apigenin, alkyl 5-nonadecyl resorcinol, 5-unacyl resorcinol, 5-tricosyl resorcinol of resorcinols, sugar pentosan, few A mixture of tettosan,...

Embodiment 3

[0023] Utilize bran to prepare the method for fungicide, its step is as follows:

[0024] 1. Ingredients: mix sugar: water = 1:15 until sugar is dissolved in water to form sugar water; Zygomyces bisporus: Bayer combined yeast: Acetobacter xylinum = 3:5:5 to form a mixed bacteria liquid;

[0025] 2. Fermentation: Mix the sugar water and the mixed bacterial solution in a ratio of 90:1 to form a mixed solution, put the bran into the fermentation tank layer by layer, and spray the mixed solution evenly after each layer until the layer is wet; keep the temperature at 28°C , fermented for 18 days;

[0026] 3. Preparation of bactericide: filter the fermented feed liquid to obtain fermented liquid and solid slag, add sodium hydroxide to the obtained fermented liquid to adjust the pH value to 7, and filter again to obtain flavonoid-containing apigenin, alkyl 5-nonadecyl resorcinol, 5-unacyl resorcinol, 5-tricosyl resorcinol of resorcinols, sugar pentosan, few A mixture of tettosan, ...

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PUM

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Abstract

The invention discloses a method for preparing a bactericide from bran, which comprises the following steps: dissolving sugar in water to form a sugar solution; mixing Saturnospora dispora, Zygosaccharomyces bailii and Acetobacter xylinum to form a mixed bacterium solution; mixing the sugar solution and bacterium solution to form a mixed solution, and putting the bran into a fermenting tank layer by layer, wherein the mixed solution is uniformly sprayed on each layer until the layer is moistened; fermenting at constant temperature, filtering the fermented liquor to obtain fermentation liquor and solid residues, adding sodium hydroxide into the obtained fermentation liquor to regulate the pH value, and filtering to obtain a mixture containing apigenin (belonging to flavones), 5-nonadecyl resorcinol, 5-heneicosyl resorcinol and 5-tricosyl resorcinol (belonging to alkyl resorcinols), and pentosan, chitosan, oligosaccharide, amino oligosaccharide, lentinan and pujuxitang; and preparing the plant-source agricultural bactericide. The preparation technique is simple and suitable for large-scale production; and the plant-source agricultural bactericide has the advantages of high safety, environmental protection and no pesticide residue.

Description

technical field [0001] The invention relates to a method for preparing a fungicide, in particular to a method for preparing a fungicide from bran. Background technique [0002] Due to the long-term use of chemical pesticides, the excess of various chemical pesticide residues has seriously affected people's health, and the research and development of environmentally friendly biological pesticides has become the main task of current pesticide science. [0003] The utilization of bran is very wide, also has report in the production of insecticide, as the production and the use method of the Bacillus thuringiensis solid fermentation product disclosed in Wang Tao (application number: 97109229.X); Tao Yugui's biopesticide solid fermentation condition In relevant reports such as studies, all describe the method of using bran as a medium, adding Bacillus thuringiensis for microbial fermentation, and producing insecticides. [0004] However, there is no report on the method of using...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N43/16A01N31/16A01P3/00
Inventor 杜圣艳
Owner 安徽省淮北市农药厂
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