Culture solution of nitrosobacteria as well as preparation method and culture method thereof

Abstract

The invention relates to a culture solution of a typical nitrosomonaseuropaea and a culture method thereof. The culture method comprises the steps of preparing the culture solution, activating the bacteria and culturing. By adopting the culture solution and the method, the outstanding problems that the existing bacteria is difficult to culture, the requirement is high, the bacteria is easy to be polluted in the culture process and slow to grow, and the like can be solved. The culture solution comprises nutritional substances needed by the growth of the bacteria, a biological buffering agent and a pH indicating agent, and the culture solution can promote the growth of the bacteria, improve the propagation capacity and survival capacity of the bacteria and can judge and adjust the growth state and activity of the bacteria according to the color variation of the indicating agent; in the culture process, the bacteria is activated, so that the activity and the growth rate of the bacteria can be further increased, the culture period can be greatly shortened, the pH can be monitored and adjusted in real time, the survival capacity of the bacteria can be further improved, and the culture efficiency can be improved.

Description

technical field [0001] The invention belongs to the field of environmental engineering microorganisms, in particular to a culture solution and a culture method of a typical ammonia oxidizing bacterium (N. europaea). Background technique [0002] At present, the nitrogen and phosphorus removal process of domestic sewage is mainly removed through the absorption and transformation of microorganisms. The ammonia nitrogen in the sewage is first oxidized into NO sequentially under the action of ammonia oxidizing bacteria and nitrosifying bacteria. 2- and NO 3- , then NO 3- Converted to N under the action of denitrifying bacteria 2 be removed. [0003] N.europaea is a typical and widespread chemoautotrophic ammonia-oxidizing bacteria in sewage biological denitrification treatment systems, and is very sensitive to environmental changes and toxic stress of pollutants. The general pathway of oxidative metabolism in the nitrogen cycle mainly includes the sequential oxidation of amm...

AI Technical Summary

Problems solved by technology

[0004] At present, due to the high sensitivity of this strain to environmental changes, the cultivation of pure strains is difficult and demanding, and problems such as slow bacterial growth, low density, low activity, and easy contamination by bacteria often occur during the cultivation process. , and even the situation that it cannot be cultivated for a long time

At the same time, the traditional culture solution formula lacks the trace mineral elements required for bacterial growth, or the concentration ratio of the nutrient elements contained is biased, and its buffer capacity is weak, which limits the adaptability, viability and reproductive ability of bacteria to a certain extent.