Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A human low-invasive bladder cancer cell

A bladder cancer cell, invasive technology, applied in the fields of biology and oncology, can solve the problems of non-expression, inconsistent cell line characteristics and genetic background, poor tumorigenicity, etc.

Active Publication Date: 2018-04-06
许传亮 +4
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] As far as the research field of bladder cancer is concerned, although bladder cancer cell lines have been established at present, due to their early establishment, their passage status is unstable and their tumorigenicity is poor, and the characteristics and genetic background of the cell lines are also inconsistent, resulting in The expression of bladder cancer-specific markers is uneven or even non-existent, and the existing cell lines do not clearly classify the degree of invasion of cancer cells, so the commonly used bladder cancer cell lines can no longer meet the requirements of modern scientific research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A human low-invasive bladder cancer cell
  • A human low-invasive bladder cancer cell
  • A human low-invasive bladder cancer cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] The establishment of embodiment 1CH-1 cell line

[0093] (a) Source:

[0094] Stage: T1

[0095] Chief complaint: hematuria

[0096] Cystoscopy: Bladder trigone 1×1cm space occupying

[0097] Pathology: high-grade urothelial carcinoma

[0098] Surgery: bladder cancer resection

[0099] (b) Primary cell culture:

[0100] Take 0.5 × 0.5 cm of cancer tissue for primary cell culture. For the method, please refer to the general method.

[0101] The fresh bladder cancer tissue digested with collagenase can grow adherently about 5 hours after centrifugation and plating. A large number of cell clones can appear in about 5 days, and mesenchymal cells and epithelial cells can be seen mixed growth at this time. The mesenchymal cells can be basically cleared after three passages by differential digestion.

[0102] (c) Animal model establishment:

[0103] The cells were taken and inoculated into immunodeficient mice. For the first time, CH-1 cell line was planted in 3 femal...

Embodiment 2

[0104] Example 2 Biological Characteristics of Low Invasive Bladder Cancer Cell Line CH-1

[0105] Morphological observation: the cell line is spindle-shaped or polygonal ( figure 1 ), the cell shape is stable.

[0106] Proliferation activity: CCK-8 reagent was used to detect the proliferation activity of CH-1 cell line, and it was found that its doubling interval was 48-72 hours ( figure 2 ).

[0107] Passage activity: The cell line has been passed to the 86th passage, the cell shape and growth rate remain unchanged, and the recovery is good after cryopreservation.

[0108] Related protein expression and marker expression of low-invasive bladder cancer:

[0109] a. Immunohistochemical method with mouse anti-human keratin antibody CK AE1 To detect the source and species of mouse tumor in situ. The results showed that patients with urothelial carcinoma of the urinary tract highly expressed CK AE1 protein, but no expression in interstitial tissue ( Figure 4 A). Orthoto...

Embodiment 3

[0111] Example 3 Study on the transfer rate and tumor formation rate of the low-invasive bladder cancer cell line CH-1

[0112] The cells were inoculated into immunodeficient mice. For the first time, the CH-1 cell line was planted in 40 female nude mice, and 35 nude mice were successfully tumor-bearing, and 19 of them had metastasis to lymph nodes or other organs. The above experiment was repeated three times, and the average tumor-bearing success rate and metastasis rate were taken. It was found that the tumor-bearing success rate of the modified cell line was about 75-80%, and the metastasis rate was lower than 60%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a human low-invasive bladder cancer cell line, specifically, a new human low-invasive bladder cancer cell line CH‑1, which was preserved in the China Center for Type Culture Collection on August 26, 2013 ( CCTCC, China, Wuhan), the deposit number is CGMCC No.C2013128. The cell line of the present invention has stable cell morphology and passage characteristics, good tumorigenicity, and the cell line does not appear distant metastasis, can be used for the establishment of animal models, and can be used in combination with multi-organ metastatic bladder cancer cell lines for screening Drugs for different properties of bladder cancer, and for screening genes related to metastasis.

Description

technical field [0001] The invention belongs to the fields of biology and oncology, and in particular, the invention relates to the establishment and application of a new low-invasive bladder cancer cell. Background technique [0002] The occurrence and development of various human diseases are very complicated, and in-depth exploration of the pathogenesis and therapeutic mechanism of various diseases cannot be carried out on patients. However, it is possible to follow the Animal Protection Law, conduct research on various diseases and life phenomena through animals, and then deduce it to human beings to explore the mysteries of human life and the laws of the occurrence and development of human diseases, so as to control the occurrence and development of human diseases, cure diseases and Reduce the suffering of human beings, improve the quality of life, and prolong the life of human beings. [0003] For a long time, people have found that it is difficult to promote the deve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09A01K67/027A61K49/00C12Q1/6886C12Q1/02
Inventor 许传亮徐伟东韦荣超吴承耀孙颖浩
Owner 许传亮
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com