Plant and AM (arbuscular mycorrhiza) fungus symbiotic bidirectional cultivating box

Abstract

Disclosed is a plant and AM (arbuscular mycorrhiza) fungus symbiotic bidirectional cultivating box. Nutrient solution is filled in a liquid storage tank, a support plate is connected above the liquid storage tank, imbibing paper and a hypha filter membrane are paved on the support plate, two ends of the imbibing paper are immersed in the nutrient solution, the upper portion of the support plate is provided with a plant growth chamber, mycorrhiza chambers and liquid exchange ports are respectively arranged on two sides of the plant growth chamber, cultivating matrix is disposed in the plant growth chamber and communicated with the bottom of the mycorrhiza chambers, and the top ends of the mycorrhiza chambers are sealed by breathable sealing membranes. The nutrient solution can automatically enter the mycorrhiza chambers and the plant growth chamber, so that plant roots can grow along the hypha filter membrane. Spores of the AM fungus can be inoculated to the surface of the root system after the root system enters the mycorrhiza chambers, hypha characteristics of the AM fungus and forming process of the root system symbiotic structure of host plants can be observed and plant photosynthesis and physiological and biochemical characteristics can be researched transparently and visually. The plant and AM fungus symbiotic bidirectional cultivating box realizes dual cultivation of the AM fungus and the entire plants, and provides an effective way to research the relation of the AM fungus and the plant and actions on the plant.

Description

technical field [0001] The invention relates to a plant cultivation box, in particular to a two-way cultivation box for the symbiosis of plants and AM fungi, and belongs to the field of fungal basic research. Background technique [0002] Constrained by the symbiotic characteristics of Arbuscular Mycorrhiza (AM) fungi, it has long been difficult to study them under transparent and intuitive conditions, resulting in a serious lag in research on the mycelial structure and physiological and biochemical characteristics of AM fungi. In recent years, the use of Agrobacterium rhizogenes ( Agrobacterium rhizogenes ) to induce hairy roots from plant roots, and on this basis, the double culture technique of isolated roots was successfully established (Wang Jingjing et al., 2010; Kastell et al. , 2013). This technique can realize in situ observation of the morphological development and physiological and biochemical characteristics of AM fungi in a petri dish, and is currently an eff...

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Problems solved by technology

However, this method is limited by the space of the petri dish and the nutritional conditions, and the whole plant cannot be cultivated, that is, the AM fungus cannot be studied in the case of the aboveground part of the plant and the participation of photosynthesis, and only sucrose can be added to the medium to replace photosynthesis. role, thus limiting the application of this technique in some studies, such as studying the mutual transfer of carbohydrates and minerals between fungi and aboveground parts of plants, etc.

Therefore, it is particularly important to invent a culture container that is both suitable for plant growth and AM fungal growth. At present, there is no ideal culture container for use.

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