Preparation of immune antigen and coating antigen for detection of chloramphenicol

A technology for immunizing antigens and coating antigens, which is applied in the field of preparation of immunizing antigens and coating antigens, and can solve the problems of immediate allergy, visceral damage, and destruction of normal flora on the skin surface, so as to improve the coupling ratio and specificity. Sex, improve the effect of immunogenicity

Active Publication Date: 2015-12-02
佛山市质量计量监督检测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the soft skin of the human face and rich mucosal blood vessels, the absorption of drugs is very fast, and chloramphenicol will be absorbed quickly, thereby destroying the normal flora on the skin surface and causing adverse reactions such as rashes and immediate allergies.
If you use cosmetics containing chloramphenicol for a long time, it may also cause internal organ damage

Method used

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  • Preparation of immune antigen and coating antigen for detection of chloramphenicol
  • Preparation of immune antigen and coating antigen for detection of chloramphenicol
  • Preparation of immune antigen and coating antigen for detection of chloramphenicol

Examples

Experimental program
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Embodiment Construction

[0021] 1 Synthesis of chloramphenicol hapten by acetal method

[0022] Add 200 mL of cyclohexane into a 250 mL round-bottomed flask equipped with a water trap, add chloramphenicol (32.3 g, 0.1 mol), 6-oxohexanoic acid (15.6 g, 0.12 mol) and methylsulfonate in sequence Acid (0.95g, 0.01mol), heating to reflux, as the reaction proceeds, the water produced by the reaction sinks at the bottom of the water separator, continue to heat and reflux until no new reaction water settles in the water separator, stop heating. Naturally cool the reaction solution to room temperature and let stand overnight. After suction filtration, the filter cake was washed twice with cyclohexane, and dried at 50° C. for 5 hours to obtain 40.4 g of carboxyl-containing chloramphenicol derivatives. The prepared chloramphenicol hapten has a structure shown in formula (I):

[0023]

[0024]

[0025] 2 Synthesis of Chloramphenicol Immune Antigen by Active Thioester Method

[0026] Accurately weigh 1.0 ...

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Abstract

The invention discloses preparation of an immunizing antigen and an envelope antigen for detecting chloramphenicol. The preparation method is characterized by comprising the following steps: by an active thioester method, coupling a chloramphenicol hapten and carrier protein, and preparing the chloramphenicol immunizing antigen and envelope antigen, wherein the chloramphenicol hapten is prepared by introducing a spacer into chloramphenicol through an acetal method. The coupling technical route is optimized, so that the structure is optimized, the antibody quality is improved and the sensitivity and specificity of rapid chloramphenicol immune colloidal gold detection are further improved with the immunizing antigen and the envelope antigen.

Description

technical field [0001] The invention relates to the technical field of chloramphenicol detection in food and cosmetics, in particular to the preparation of immune antigens and coating antigens for detection of chloramphenicol. Background technique [0002] Chloramphenicol is a broad-spectrum antibiotic with good antibacterial effect and low price. It has been widely used in the prevention and treatment of animal diseases. However, chloramphenicol has serious toxic and side effects, which can cause the imbalance of normal flora in animals, and may cause diseases such as aplastic anemia and agranulocytosis in humans. [0003] EU 2377 / 90 / EEC regulations, US FDA, etc. all stipulate that chloramphenicol cannot be used in any animal food, animal feed and growth environment. In 2002, the Ministry of Agriculture of my country issued Announcement No. 235 "The Maximum Residues of Veterinary Drugs in Animal Food", which stipulated that chloramphenicol and its salts and esters (includi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/531G01N33/68
Inventor 潘嘉慧田峻赵亮亮沈国权朱海云杨辉
Owner 佛山市质量计量监督检测中心
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