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Culture medium for promoting hybridoma growth and preparation method thereof

A culture medium and hybridoma technology, applied in biochemical equipment and methods, tissue culture, microorganisms, etc., can solve the problems of strengthening the ability of hybridoma cells to secrete specific antibodies, complex preparation methods, and inconvenient operation

Inactive Publication Date: 2014-06-18
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing medium only focuses on promoting the growth of hybridoma cells, but fails to enhance the ability of hybridoma cells to secrete specific antibodies at the same time, and the preparation method is relatively complicated, and the operation is not easy enough

Method used

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  • Culture medium for promoting hybridoma growth and preparation method thereof
  • Culture medium for promoting hybridoma growth and preparation method thereof
  • Culture medium for promoting hybridoma growth and preparation method thereof

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Embodiment Construction

[0013] Hybridoma cells are produced by the fusion of B cells and myeloma cells, and have the characteristics of both B cells and myeloma cells. Lymphocyte hybridoma technology is an important technology in the process of producing monoclonal antibodies. However, using this technology to prepare monoclonal antibodies requires many experimental procedures, long cycles, complex influencing factors, and easy setbacks. Especially in the early stage of hybridoma production, because the emerging hybridoma has not yet adapted to the tissue culture conditions in vitro, it is easy to die prematurely or cannot secrete specific antibodies well.

[0014] Aiming at the above problems, this example designs a cell culture medium containing the cytokine IL-21 (IL-21) that can promote the proliferation and differentiation of B cells and the production of specific antibodies, which overcomes the conventional new generation of hybridoma cells in vitro Difficulty in cultivating premature death or ...

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Abstract

The invention relates to a culture medium for promoting hybridoma growth and a preparation method thereof. The culture medium for promoting hybridoma growth comprises a 1640 complete medium and recombinant human IL-21 added into the 1640 complete medium, and the concentration of recombinant human IL-21 in the medium for promoting hybridoma growth is 100 ng / mL. According to the above culture medium, the recombinant human IL-21 is added on the basis of a prepared RPMI 1640 complete medium, and the final concentration of recombinant human IL-21 in the culture medium is controlled at 100 ng / mL, so that the growth of hybridoma cells is promoted by promoting B cell proliferation, and also secretion of needed monoclonal antibody by the hybridoma cells is promoted by promoting B cell to secrete specific antibody.

Description

technical field [0001] The cell culture medium and its preparation method, more specifically relate to a culture medium capable of providing better growth conditions for hybridoma cells and its preparation method. Background technique [0002] With its high specificity and sensitivity, monoclonal antibodies are widely used in the fields of immunological detection, affinity purification of target proteins and tumor treatment. Lymphocyte hybridoma technology is an extremely important link in the process of producing monoclonal antibodies. However, using this technology to prepare monoclonal antibodies requires many experimental procedures, long cycles, complex influencing factors, and easy setbacks. Especially in the early stage of hybridoma production, due to the poor viability of newly emerging hybridomas, their growth conditions are likely to change due to environmental changes, they cannot well adapt to in vitro tissue culture conditions, and they are prone to premature de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/20C12N5/24
Inventor 万晓春张茜金言阮庆国
Owner SHENZHEN INST OF ADVANCED TECH
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