Beta-glucosidase high-yielding strain and application thereof in conversion preparation of genipin and resveratrol

Abstract

The invention discloses a beta-glucosidase high-yielding strain. The beta-glucosidase high-yielding strain is screened from the nature, with fructus gardenia as a carbon source, and has a preservation number of CCTCC No.M2013634 and is classified and named as penicillium rolfsii HXL; the strain is able to efficiently convert geniposide and polydatin into genipin and resveratrol; on that basis, a method for converting and preparing genipin and resveratrol by using the beta-glucosidase high-yielding strain above is created. The method has the advantages that geniposide and polydatin are respectively hydrolyzed under a mild condition, so as to avoid the shortage of the loss of active ingredients of the traditional method, and thus the yield is effectively increased; in addition, the active ingredients, namely, geniposide or polydatin, of fructus gardenia or polygonum cuspidatum are converted by mass under an in-vitro controllable condition by the modern microbial fermentation engineering technology and enzyme engineering technology, and thus the production cost is decreased, and the production efficiency is improved. With the adoption of the beta-glucosidase high-yielding strain, a feasible manner is provided for industrially producing beta-glucosidase, genipin and resveratrol as mass, large economic benefit and social benefit are brought, and the market development prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of microbial biotransformation, and in particular relates to a β-glucosidase high-yielding bacterium and its application in transforming and preparing genipin and resveratrol. Background technique [0002] Genipin is a kind of iridoid substance, and it is an important component that plays the main medicinal effect in Crimson chinensis. Genipin has a significant effect on the treatment of liver cirrhosis, and also has significant anti-inflammatory, anti-lipid peroxidation, anti-angiogenesis and other effects. Genipin can also be applied to the cross-linking of biological materials. It can not only form stable Cross-linked products have the advantages of low cytotoxicity, good biocompatibility, strong degradation resistance and wide application fields, and are very promising bio-cross-linking agents. The content of genipin in gardenia is very low (about 0.005% to 0.01%), and it is very difficult to extract di...

AI Technical Summary

Problems solved by technology

Although the β-glucosidase gene has been successfully cloned and expressed, and the newly constructed engineering bacteria have also been applied to production practice, the enzymatic hydrolysis of extracted geniposide and resveratrol glycosides by β-glucosidase is still There are many shortcomings, such as: complicated process and high cost, which limit its further development

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