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Porphyra haitanensis polysaccharide positioning sulphating method

A technology of sulfated esterification and seaweed polysaccharide, which is applied in the fields of polysaccharide derivatization and marine medicine, can solve the problems of high viscosity, limited application, poor solubility, etc., and achieves the effects of simple operation, low manufacturing cost and stable preparation process.

Inactive Publication Date: 2014-06-18
张忠山
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the high molecular weight of laver polysaccharides in the altar, its solubility in cold water is extremely poor, and its viscosity is very high, so its application is limited.

Method used

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  • Porphyra haitanensis polysaccharide positioning sulphating method
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  • Porphyra haitanensis polysaccharide positioning sulphating method

Examples

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Embodiment 1

[0032] 1. Preparation of Porphyra polysaccharide

[0033] Add 40 times of water to dry altar seaweed and extract at 120°C for 3 hours. The extract is filtered with diatomaceous earth and placed in a dialysis belt. Dialyzed with running tap water for two days and distilled water for one day. After precipitation and drying, the raw material polysaccharide of laver was obtained, which was defined as sample 1.

[0034] 2. Alkali treatment of Porphyra polysaccharide

[0035] Weigh 1.6g Porphyra laver crude polysaccharide and dissolve it in 250mL water, treat it with 0.2g potassium borohydride at room temperature for 48h, then add 10g sodium hydroxide and 3.0g potassium borohydride to the solution, seal the flask gently, 2h in a water bath. The lye was neutralized with acetic acid, dialyzed, and freeze-dried to obtain the alkali-treated polysaccharide, which was defined as sample 2.

[0036] 3. Full sulfation of Porphyra polysaccharides

[0037] Place a three-necked flask contai...

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Abstract

The invention belongs to the technical field of polysaccharide derivatization of the marine drug field, in particular relates to a porphyra haitanensis polysaccharide positioning sulphating method which comprises steps of totally sulfating porphyra haitanensis polysaccharide, preparing 6-O-sulfated porphyra haitanensis polysaccharide, preparing 2,2',4-O-sulfated porphyra haitanensis polysaccharide, and the like. High molecular weight polysaccharide obtained from water extract and alcohol precipitate is used as a raw material, hydroxyl at a specific part of the polysaccharide is protected, and subsequently sulphating derivatives of different parts are synthesized. A modified product provided by the invention is good in solubility, has different anticoagulation effects in in-vitro anticoagulation experiment, and is applicable to different medical demands.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide derivatization in the field of marine medicine, and in particular relates to a method for positioning sulfation of polysaccharides from Porphyra laver. Background technique [0002] Seaweed polysaccharide is a kind of multi-component mixture, which is composed of different monosaccharide groups connected by glycosidic bonds (generally 1→3 and 1→4 bonds). It is a variety of high molecular carbohydrates contained in seaweed cells and cells. The general term for compounds. It is generally water-soluble, most of which contain sulfuric acid groups, and most of them have high viscosity or coagulation ability. Although many marine bioactive polysaccharides have been discovered so far, few of them have been directly developed into drugs from their primary extracts, because the biological activity of seaweed polysaccharides is often limited by its structure. However, by introducing certain chemica...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/737A61P7/02
Inventor 张忠山王晓梅张旭
Owner 张忠山
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