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A compound of phycocyanin and phthalocyanine and its preparation method and application

A technology of phycocyanin and compound, which is applied in the direction of botanical equipment and methods, applications, medical preparations containing active ingredients, etc., can solve the problems of easy aggregation of zinc, increased biological activity, poor water solubility, etc., and overcome hydrophobicity , good water solubility effect

Active Publication Date: 2016-01-20
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, phthalocyanine also has some disadvantages such as easy aggregation of zinc phthalocyanine in physiological solution, poor water solubility and biological activity to be further improved.

Method used

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  • A compound of phycocyanin and phthalocyanine and its preparation method and application
  • A compound of phycocyanin and phthalocyanine and its preparation method and application

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preparation example Construction

[0032] The preparation method of the complex of phycocyanin and phthalocyanine according to the present invention comprises the following main steps: (a) dissolving the phthalocyanine complex in a suitable solvent, preparing a certain concentration of the phthalocyanine complex solution, and dissolving Water-soluble phycocyanin is dissolved in buffer solution or buffer solution containing urea to prepare a certain concentration of water-soluble phycocyanin solution; (b) According to a certain feeding ratio, gradually add the phthalocyanine complex solution to the water-soluble phycocyanin solution to form a reaction mixture; (c) shake or stir the above reaction mixture at 25-37°C for 2-12 hours, so that the phthalocyanine complex and water-soluble phycocyanin Combined into a complex to obtain a mixed solution containing the complex and free phthalocyanine complexes; (d) separate the above solution by gel permeation chromatography with a buffer solution as an eluent; (e) separat...

Embodiment 1

[0040] Extraction and Purification of Phycocyanin

[0041] (1) Crude extraction of phycocyanin

[0042] Prepare a phosphate buffer solution with pH=7, weigh 10g of spirulina powder, dissolve it in 250mL of buffer solution, oscillate ultrasonically for 5 minutes, and place it in a -20°C refrigerator for 12 hours. Its electronic absorption spectrum.

[0043] (2) Salting out of phycocyanin

[0044] Take the phycocyanin water extract, add saturated ammonium sulfate solution or ground ammonium sulfate powder, make the saturation of ammonium sulfate in the solution respectively 40%, 4°C overnight, carry out salting out, centrifuge at 12000r / min for 20min, and put the above Dilute the supernatant to an appropriate multiple or dissolve the precipitate with an appropriate amount of distilled water, then measure the UV-visible absorption spectrum, and measure the wavelength range of 250-800nm ​​to calculate the purity and concentration of the protein in the solution.

[0045] (3) Desal...

Embodiment 2

[0051] Synthesis of 1,8(11), 15(18), 22(25)-tetrakis(3-carboxyphenoxy)zinc phthalocyanine

[0052] (1) Preparation of intermediate 3-(3-carboxyphenoxy)phthalonitrile:

[0053] Add 0.69g (5mmol) 3-hydroxybenzoic acid and 0.69-2.08g (4-6mmol) (preferably 0.87g, 5mmol) 3-nitrophthalonitrile into 35ml DMF, blow nitrogen, stir at room temperature, after 10 minutes Add 2.1g (15mmol) of potassium carbonate and react for 24 hours. Suction filter the reaction mixture, collect the filtrate, add 200ml ice-water mixture to the filtrate, adjust the pH value to 1~3 (preferably 2) with 2mol / L HCl solution, the product precipitates out, filter, wash with water until neutral, collect and filter The cake was dried under normal pressure at 70°C to obtain a crude product. The crude product was dissolved with a small amount of DMF, then a large amount of water was added to precipitate a precipitate, filtered, washed with water three times, and dried under normal pressure at 70°C to obtain 1.11 g...

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Abstract

The invention discloses a complex and its preparation methods and applications of algae -blue protein and 酞 酞. It is compounded on the algae blue protein to compound the pupae molecules through non -covalent.The composition ratio of things.The compound of algae blue protein and 的 的 As a photoresist with a dual -functional base and a spectrum of action spectrum, it has the advantages of high biocompatibility and high optical anti -cancer activity.

Description

technical field [0001] The invention belongs to the field of functional materials and medicines, and in particular relates to a compound of phycocyanin and phthalocyanine, a preparation method and application thereof. Background technique [0002] Phycocyanin is a porphyrin-like pigment protein with good water solubility; it is a natural protein derived from spirulina. Composed of equal moles of α and β subunits, the α and β subunits first form monomers (αβ) through various interactions between molecules, and the monomers are polymerized into trimers (αβ) with the help of linking polypeptides 3 and hexamers (αβ) 6 . [0003] Phycocyanin contains only one chromophore, that is, phycocyanobilin (PCB). Each subunit is composed of an apoprotein and a chromophore with an open-chain tetrapyrrole structure, and the chromophore is cross-linked with the cysteine ​​residue of the apoprotein through a thioether bond. The α subunit contains one chromophore, while the β subunit contai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61P35/00A61P17/00A61P31/00A61P1/02A61P27/02A61P9/10A01N65/03A01P1/00A01N43/90
Inventor 黄剑东段继英万东华郑碧远郑永权
Owner FUZHOU UNIV
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