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Application of rna polymerase ii fifth subunit regulatory protein in preparation of reagents for hepatocellular carcinoma prognosis or auxiliary tace prognosis

A technology of RNA polymerase and protein regulation, applied in the field of medical bioengineering, can solve problems such as unclear RMP correlation

Active Publication Date: 2015-09-09
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the correlation between RMP and adjuvant TACE after liver cancer surgery is currently unclear in the art

Method used

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  • Application of rna polymerase ii fifth subunit regulatory protein in preparation of reagents for hepatocellular carcinoma prognosis or auxiliary tace prognosis
  • Application of rna polymerase ii fifth subunit regulatory protein in preparation of reagents for hepatocellular carcinoma prognosis or auxiliary tace prognosis
  • Application of rna polymerase ii fifth subunit regulatory protein in preparation of reagents for hepatocellular carcinoma prognosis or auxiliary tace prognosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the expression of RMP in liver cancer tissue samples

[0044] A total of 278 surgically resected samples of hepatocellular carcinoma and 20 samples of portal vein tumor thrombus were selected from Dongfang Hepatobiliary Surgery Hospital. The characteristic parameters of 278 samples are shown in Table 1.

[0045] Table 1. Characteristic parameters of patients in the test group

[0046]

[0047] Using a polyclonal rabbit-derived RMP antibody (1:75, Proteintech), the expression level of RMP protein in tissues was detected by immunohistochemistry. The immunohistochemical method comprises the steps of detecting the expression level of RMP in paraffin-embedded hepatocellular carcinoma and adjacent normal liver tissues after surgical resection of primary hepatocellular carcinoma patients by immunohistochemical method. For antigen retrieval for 2 minutes, the primary antibody was incubated with polyclonal rabbit-derived RMP antibody 1:75, proteintech, and inc...

Embodiment 2

[0053] Embodiment 2, Kaplan-Meier method comparison RMP high and low expression postoperative survival time

[0054] According to the IRS score, the samples were divided into RMP high expression group and low expression group (according to the immunohistochemical staining intensity score, 0,1 points were defined as low expression; 2,3 points were defined as high expression), as shown in Table 2.

[0055] Table 2. Correlation between RMP protein expression and clinicopathological characteristics in the test group (n=278)

[0056]

[0057]

[0058] The P value in Table 2 indicates that the expression level of RMP was significantly correlated with gender, the expression level of RMP was significantly correlated with venous invasion, the expression level of RMP was significantly correlated with tumor size, the expression level of RMP was significantly correlated with BCLC stage, and the expression level of RMP was significantly correlated with BCLC stage. Significantly corre...

Embodiment 3

[0063] Example 3. Different RMP expression levels can determine the curative effect of postoperative adjuvant TACE

[0064] In the patients in Example 2, the postoperative implementation of adjuvant TACE was collected, stratified according to the RMP immunohistochemical score, and the prognostic survival curve was drawn using SPSS18.0 analysis software. The results are as follows image 3 A-B.

[0065] The results showed that in the patients with high expression of RMP, postoperative adjuvant TACE can significantly prolong the survival time of patients, and the curative effect is significantly better than that of the control group; while in patients with low expression of RMP, postoperative adjuvant TACE does not improve patient outcomes.

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Abstract

The invention relates to an application of an RNA (Ribose Nucleic Acid) polymerase II fifth subunit regulatory protein (RMP) in preparation of a reagent for prognosis of hepatocellular carcinoma or auxiliary TACE (Transcatheter Arterial Chemoembolization). The invention firstly reveals that high expression of the RMP in a hepatic cell cancer indicates unsatisfactory prognosis of resection of hepatocellular carcinoma after operation; meanwhile, the high expression of the RMP further indicates that the auxiliary TACE after operation is effective. Thus, the invention establishes an RMP-based prognosis method of the hepatic cell cancer after operation.

Description

technical field [0001] The invention belongs to the technical field of medical bioengineering; specifically, the invention relates to the application of RNA polymerase II fifth subunit regulatory protein (RMP) for evaluating the poor prognosis of primary hepatocellular carcinoma and the curative effect of auxiliary TACE . Background technique [0002] Primary liver cancer is one of the most common clinical malignant tumors. China is a high-incidence area for primary liver cancer. About half of the world's new cases occur in China every year. Hepatocellular carcinoma (HepatoCellular Carcinoma, HCC) is a common pathological type of primary liver cancer, accounting for about 70-85%. Surgical resection is currently the treatment of choice for primary liver cancer. However, the high recurrence rate after surgical resection of primary liver cancer is still the main factor leading to the poor prognosis of liver cancer patients. Adjuvant transcatheter arterial chemoembolization (...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/57438G01N33/57484G01N33/68
Inventor 王红阳董立巍张建谈冶雄
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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