Colloidal gold reagent strip and method for rapidly detecting Boule protein

Abstract

The invention discloses a colloidal gold reagent strip for rapidly detecting Boule protein. A sample pad, a gold label pad, a chromatography film and a water absorption pad of which the ends are sequentially connected with one another are arranged on a rubber plate according to the sample flow direction; a detection area T and a quality control area C are formed in the chromatography film; the sample refers to supernatant of lysate of testicular tissue to be detected; a colloidal gold-anti-Boule protein polyclonal antibody-1 conjugate is adsorbed to the gold label pad; an anti-Boule protein polyclonal antibody-2 is coated in the detection area T; an anti-rabbit IgG is coated in the quality control area C. The detection method comprises the following steps: 1, taking the supernatant of lysate of testicular tissue to be detected; 2, dripping 2-3 droplets of the supernatant of lysate of testicular tissue to be detected on the sample pad; and 3, observing the result within 5 minutes. According to the reagent strip, the existence of the Boule protein in the lysate of the testicular tissue is detected by adopting a double antibody sandwich method and an immunochromatographic method. The detection process is high in sensitivity, high in specificity and high in accuracy, the sperm development block condition is clear at a time, the detection speed is high, the result can be obtained within 5 minutes, and the operation is simple.

Description

technical field [0001] The invention relates to a colloidal gold reagent strip and a detection method, in particular to a colloidal gold reagent strip and a detection method for rapidly detecting Boule protein. Background technique [0002] my country is currently facing increasingly serious infertility problems. The survey shows that 10% of married families in China are deeply troubled by infertility, and the proportion is increasing year by year. 50% of infertility is caused by male infertility factors. Environmental problems, abnormal metabolism of the body and genetic defects can all lead to male infertility. The deep-rooted concept of family inheritance has brought a great mental burden to male infertility patients. The problem of male infertility not only threatens all aspects of family life, but also affects the harmony of the whole society, becoming an unstable factor in society. It is imminent to improve the diagnosis and treatment rate of male infertility. [000...

AI Technical Summary

Problems solved by technology

However, there is no rapid and effective detection method for Boule gene at present.

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