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Fusarium oxysporum and application of same in degrading autotoxins resulting in successive cropping obstacles

A technology of Fusarium oxysporum and continuous cropping obstacles, which is applied in the field of Fusarium oxysporum and its application in the degradation of self-toxic substances of continuous cropping obstacles. problem, to achieve the effect of simple method of use, low cost and broad application prospects

Inactive Publication Date: 2014-06-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This strain has a certain degradability to benzoic acid, but the degradation efficiency of this strain to benzoic acid is not recorded, and it is not known whether this strain has a degrading effect on self-toxic substances other than benzoic acid, and it cannot effectively eliminate the effects caused by self-toxic substances. Continuous cropping obstacle

Method used

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  • Fusarium oxysporum and application of same in degrading autotoxins resulting in successive cropping obstacles
  • Fusarium oxysporum and application of same in degrading autotoxins resulting in successive cropping obstacles
  • Fusarium oxysporum and application of same in degrading autotoxins resulting in successive cropping obstacles

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Embodiment Construction

[0029] 1 Culture medium and solution preparation

[0030] (1) Inorganic salt culture medium: NaCl0.5g, MgSO 4 ·7H 2 O0.2g, NH 4 NO 3 1.0g, KHPO 4 1.0g, K 2 HPO 4 1.0g, GaCl 2 2H 2 O0.02g, FeCl 3 ·6H 2 O0.05g, add 1000mL of distilled water, stir evenly, adjust the pH value to 7.0, and sterilize under high pressure steam at 121°C for 20min.

[0031] (2) Beef extract-peptone medium: beef extract 10g, peptone 5.0g, sodium chloride 5.0g, distilled water to make up to 1000mL, mix and stir evenly, adjust the pH value to 7.0, and autoclave at 121°C for 20min.

[0032] (3) Red Bengal medium: KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, peptone 5g, glucose 5g, agar 16g, Bengal red 0.053g, distilled water 1000mL, adjust the pH value to 7.0, autoclave at 121°C for 20min; add 30μg / mL chloramphenicol before use.

[0033](4) PDA medium: Wash and peel the potatoes, then weigh 200g of potatoes and cut them into small pieces, boil them in water (boil for 20-30 minutes, it can be pierced ...

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Abstract

The invention discloses Fusarium oxysporum and application of the same in degrading autotoxins resulting in successive cropping obstacles. The Fusarium oxysporum has an accession number of CCTCC No. M2013432. The Fusarium oxysporum can use autotoxins as sole carbon source and energy for growth, which belongs to mineralization, and the Fusarium oxysporum has positive meaning to removal of autotoxins in a cultivation water body or soil and overcoming of successive cropping obstacles. As the Fusarium oxysporum has grown for 72 h with autotoxins as sole carbon source and energy, a highest degradation rate of cinnamic acid reaches 99.53%, a highest degradation rate of benzoic acid reaches 97.9%, a highest degradation rate of vanillin reaches 92.5%, a highest degradation rate of p-hydroxybenzoic acid reaches 95.6%, and no side effects are exerted on crops in degradation of autotoxins. Application of the Fusarium oxysporum is simple and convenient and has low cost and wide application prospects.

Description

technical field [0001] The invention belongs to the field of development and utilization of microbial germplasm resources, and in particular relates to a Fusarium oxysporum and its application in degrading continuous cropping obstacle autotoxic substances. Background technique [0002] Autotoxic substances refer to the chemical substances released by plants through aboveground leaching, root exudation, and plant residue decomposition, which inhibit the growth of the same species or the same family of plants in the same stubble or the next stubble. The autotoxic substances accumulated in cucumber soil were mainly composed of more than ten kinds of phenolic compounds including cinnamic acid, benzoic acid and salicylic acid. On the one hand, these substances affect plant growth by affecting ion absorption, water absorption, photosynthesis, protein and DNA synthesis and other pathways, and more importantly, the accumulation of autotoxic substances in rhizosphere soil provides a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01B79/00B09C1/10C12R1/77
Inventor 喻景权宋刘霞叶素芬周艳虹师恺夏晓剑
Owner ZHEJIANG UNIV
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