Fusarium oxysporum and application of same in degrading autotoxins resulting in successive cropping obstacles
A technology of Fusarium oxysporum and continuous cropping obstacles, which is applied in the field of Fusarium oxysporum and its application in the degradation of self-toxic substances of continuous cropping obstacles. problem, to achieve the effect of simple method of use, low cost and broad application prospects
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[0029] 1 Culture medium and solution preparation
[0030] (1) Inorganic salt culture medium: NaCl0.5g, MgSO 4 ·7H 2 O0.2g, NH 4 NO 3 1.0g, KHPO 4 1.0g, K 2 HPO 4 1.0g, GaCl 2 2H 2 O0.02g, FeCl 3 ·6H 2 O0.05g, add 1000mL of distilled water, stir evenly, adjust the pH value to 7.0, and sterilize under high pressure steam at 121°C for 20min.
[0031] (2) Beef extract-peptone medium: beef extract 10g, peptone 5.0g, sodium chloride 5.0g, distilled water to make up to 1000mL, mix and stir evenly, adjust the pH value to 7.0, and autoclave at 121°C for 20min.
[0032] (3) Red Bengal medium: KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, peptone 5g, glucose 5g, agar 16g, Bengal red 0.053g, distilled water 1000mL, adjust the pH value to 7.0, autoclave at 121°C for 20min; add 30μg / mL chloramphenicol before use.
[0033](4) PDA medium: Wash and peel the potatoes, then weigh 200g of potatoes and cut them into small pieces, boil them in water (boil for 20-30 minutes, it can be pierced ...
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