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Derivant of establishing rheumatoid arthritis animal model, preparation and application thereof

An animal model, rheumatoid technology, applied in the biological field, can solve the problems of imperfect model, poor stability, poor reliability, inability to describe disease aggravation and remission, etc. Effect

Active Publication Date: 2014-06-04
四川普莱美行之生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (3) Ovalbumin-induced arthritis model, although the success rate of this model is high, it has some differences from RA in terms of immunological indicators and disease course characteristics
[0008] (4) Disease combination model, which is not yet perfect, difficult to evaluate symptom attributes, poor stability and reliability, etc., and has not been adopted by clinical basic research
Small animal models cannot well simulate the pathogenesis and severity of human RA, and cannot describe the exacerbation and remission of the disease
In addition, the existing inducers used in the construction of the CIA model cannot simulate the clinical symptoms and pathological changes of rheumatoid arthritis well due to the limited degree of immune response and the short duration of the inflammatory response.

Method used

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  • Derivant of establishing rheumatoid arthritis animal model, preparation and application thereof
  • Derivant of establishing rheumatoid arthritis animal model, preparation and application thereof
  • Derivant of establishing rheumatoid arthritis animal model, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of inducer (the preparation process is as Figure 5 Shown)

[0045] 1) Prepare glacial acetic acid solution: take glacial acetic acid, add double distilled water, and dilute to a glacial acetic acid solution with a concentration of 0.1 mol / L;

[0046] 2) Preparation of collagen solution: add type II collagen to the glacial acetic acid solution prepared in step 1), pipette evenly, and store in a refrigerator at 4°C for more than 8 hours to prepare a type II collagen solution. The type II collagen can be Type II collagen commonly used in cattle, pigs, and humans;

[0047] 3) Preparation of collagen emulsion: Add the type II collagen solution prepared in step 2) dropwise to the same volume of complete Freund's adjuvant (as an optional way, the temperature of the complete Freund's adjuvant is controlled at 4-8°C) At the same time, aluminum hydroxide was added, and quickly stirred to make it fully emulsified to obtain a mixed emulsion containing type II col...

Embodiment 2

[0048] Example 2 Preparation of inducer

[0049] 1) Prepare glacial acetic acid solution: take glacial acetic acid, add double distilled water, and dilute to a glacial acetic acid solution with a concentration of 0.1 mol / L;

[0050] 2) Preparation of collagen solution: add type II collagen to the glacial acetic acid solution prepared in step 1) to prepare a type II collagen solution with a collagen solution concentration of 4mg / ml. After pipetting evenly, store in a refrigerator at 4°C for 8 hours ;

[0051] 3) Preparation of collagen emulsion: Add the type II collagen solution prepared in step 2) dropwise to the same volume of complete Freund's adjuvant (as an optional way, the temperature of the complete Freund's adjuvant is controlled at about 4°C) At the same time, add aluminum hydroxide, stir quickly to make it fully emulsified, and obtain a mixed emulsion containing type II collagen, aluminum hydroxide, glacial acetic acid and complete Freund’s adjuvant. The final concentrati...

Embodiment 3

[0052] Example 3 Preparation of inducer

[0053] 1) Prepare glacial acetic acid solution: take glacial acetic acid, add double distilled water, and dilute to a glacial acetic acid solution with a concentration of 0.1 mol / L;

[0054] 2) Preparation of collagen solution: add type II collagen to the glacial acetic acid solution prepared in step 1) to prepare a type II collagen solution with a collagen solution concentration of 8mg / ml. After pipetting evenly, store in a refrigerator at 4°C for 12 hours ;

[0055] 3) Preparation of collagen emulsion: Add the type II collagen solution prepared in step 2) dropwise to the same volume of complete Freund's adjuvant (as an optional way, the temperature of the complete Freund's adjuvant is controlled at about 4°C) At the same time, add aluminum hydroxide, stir quickly to make it fully emulsified, and obtain a mixed emulsion containing type II collagen, aluminum hydroxide, glacial acetic acid and complete Freund’s adjuvant. The final concentrat...

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Abstract

The invention discloses a derivant of establishing a rheumatoid arthritis animal model, and preparation and application thereof; the derivant comprises II type collagen, aluminium hydroxide, glacial acetic acid and complete freund adjuvant; the derivant can successfully form the rheumatoid arthritis animal model, can substantially boost immunization reaction, can keep arthritis symptom to last for a longer time, so clinic symptom and pathology change of the animal model can be more close to real rheumatoid arthritis; the preparation method of the derivant is simple in operation, and convenient for promotion and application; the derivant is used for establishing the rheumatoid arthritis animal model, is simple and safe in realization, good in stable reliability, high in molding success rate, and has excellent application prospect.

Description

technical field [0001] The invention belongs to the field of biology, and relates to an inducer for establishing an animal model of rheumatoid arthritis and its preparation and application. technical background [0002] Rheumatoid arthritis (RA) is a chronic autoimmune disease with symmetrical polyarthritis as the main clinical manifestation. The prevalence rate in my country is 0.32%-0.36%. one. [0003] RA is a kind of invasion of multiple joints in the whole body. The pathological feature is intra-articular synovitis. In the early stage, it is synovial hyperemia, synovial membrane loosening, and chronic inflammation with a large increase in new fibrovascular tissue, resulting in irregular thickening of the synovial membrane, and hypertrophy of the villi. Pannus is formed in the joint cavity, which then erodes and destroys the joint bone cortex and cartilage tissue, eventually leading to joint ankylosis, deformity and loss of function; an irreversible damage. Its pathoge...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K39/39A61K38/39A61K9/107
Inventor 曾文龚立潘玲珍申渝波
Owner 四川普莱美行之生物科技有限公司
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