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Molecular genetic marker for slaughter performance of Bian chicken and application thereof

A technology of molecular genetic markers and slaughter performance, applied in the field of poultry breeding, can solve problems such as long cycle, low efficiency, and accelerated genetic progress

Inactive Publication Date: 2014-05-28
YANGZHOU UNIV
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AI Technical Summary

Problems solved by technology

At present, the screening method for chicken slaughter performance in breeding is mainly through traditional breeding methods, so the cycle is long and the efficiency is low. Finding a suitable DNA marker for assisted selection of slaughter performance can enhance the accuracy of selection and accelerate genetic progress

Method used

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  • Molecular genetic marker for slaughter performance of Bian chicken and application thereof
  • Molecular genetic marker for slaughter performance of Bian chicken and application thereof

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Experimental program
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Effect test

Embodiment 1

[0011] 1. Test material

[0012] The blood samples of 299 hens and 81 roosters of the 7th generation Bian chicken core group were collected from the Animal Husbandry and Veterinary Research Institute of Shanxi Academy of Agricultural Sciences. Heparin sodium was used as an anticoagulant, and 0.5 mL of blood samples were collected from the veins of the hens of the seventh-generation border chicken core group. Genomic DNA was extracted by conventional phenol-chloroform method, and the DNA concentration was determined for later use.

[0013] 2. Primer Design and PCR Amplification

[0014] 2.1 Design of enzyme digestion primers

[0015] According to the chicken MSTN gene sequence in GenBank (GenBank accession number is AF346599), a pair of primers were designed for the BbvI site of exon 1, and the size of the amplified product was 162 bp. The primer sequences are as follows:

[0016] P1: F:5'-GCATTAGCAGGGACGTTAT-3' (SEQ ID NO: 1)

[0017] R: 5'-ACTCCGTAGGCATTGTGAT-3' (SEQ ID N...

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Abstract

The invention relates to the field of poultry breeding, specifically to a molecular genetic marker for slaughter performance of Bian chicken and application thereof. The invention discloses application of a myostatin (MSTN) gene as the molecular genetic marker for slaughter performance of Bian chicken. A method for improving slaughter performance of Bian chicken by using the MSTN gene comprises the following concrete steps: carrying out amplification so as to obtain a target fragment of the MSTN gene (162 bp), subjecting the target fragment to digestion by restriction enzyme BbvI, carrying out gel electrophoresis, then carrying out silver staining for color development so as to obtain a restriction map of DNA and based on the quantity and the sizes of stripes in the restriction map, judging slaughter performance of to-be-tested samples and selecting samples according with breeding purposes, wherein samples with only 162 bp stripes are homozygous samples with high slaughter performance and samples with only 127 bp stripes are homozygous samples with low slaughter performance. The method provided by the invention is simple and fast, is not affected by an external environment and can be used for molecular genetic marking of slaughter performance of Bian chicken and for marker assisted selection.

Description

technical field [0001] The invention relates to the field of poultry breeding, in particular to a method for improving the slaughter performance of side chickens by using molecular markers. Background technique [0002] Slaughter performance is one of the most important bases to measure the performance of chicken breeds, and it is a trait that broiler breeders strive to improve. Slaughter traits are quantitative traits controlled by multiple genes. With the development of molecular biology techniques, geneticists and breeders have realized that the genes controlling quantitative traits are not randomly distributed in the genome, and there are main genes that affect quantitative traits . At present, the screening method for slaughter performance of chickens in breeding is mainly through traditional breeding methods, so the cycle is long and the efficiency is low. Finding suitable DNA markers for assisted selection of slaughter performance can enhance the accuracy of selectio...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张跟喜丁馥香王金玉张丽谢恺舟戴国俊张李俊
Owner YANGZHOU UNIV
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