Post-column derivatization type high-performance liquid chromatography
A high-performance liquid chromatography and post-column derivatization technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of uncommon use of liquid chromatography, high cost, good reproducibility, etc., and achieve reproducible results. Good reproducibility, increased surface area, and fast analysis speed
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Embodiment 1
[0020] Connect the high-performance liquid chromatograph with the peristaltic pump of the discarded liquid chromatograph, and connect it with the gas chromatograph so that the hardware conditions for post-column derivatization are equipped.
[0021] The stainless steel column filled with 10-50 μm powdered steel powder (the specific particle inner diameter is optimized and screened according to the water and substance) is used as a post-column derivation column to connect with the separation column of the high-performance liquid chromatograph, and with the waste liquid chromatograph connected with the peristaltic pump, so that the sample is preliminarily separated by the separation column and then enters the post-column derivatization column. Derivative heating is balanced to achieve the purpose of post-column derivatization.
Embodiment 2
[0023] Connect the high-performance liquid chromatograph with the peristaltic pump of the discarded liquid chromatograph, and connect it with the thermostat box, so that the hardware conditions for post-column derivatization are equipped.
[0024] The stainless steel column filled with 10-50 μm powdery sand core (the specific particle inner diameter is optimized and screened according to the water and substance) is used as a post-column derivation column to connect with the separation column of the high-performance liquid chromatography, and with the waste liquid chromatography connected with the peristaltic pump, so that the sample is preliminarily separated by the separation column and then enters the post-column derivatization column. Derivative heating is balanced to achieve the purpose of post-column derivatization.
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