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Method for screening Candidatus Liberibacter resisting chemical through catharanthus roseus

A technology of phloem bacilli and periwinkle, which is applied in the field of screening antibacterial drugs and using periwinkle to screen anti-phloem bacillus drugs, can solve the problems of no anti-phloem bacilli drug screening methods, etc., and achieve the effect of simple operation and fast screening method

Active Publication Date: 2014-05-21
GUANGXI UNIV
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Problems solved by technology

[0004] After searching, at present, there is no relevant literature report on the screening method of anti-phloem bacillus drug

Method used

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  • Method for screening Candidatus Liberibacter resisting chemical through catharanthus roseus
  • Method for screening Candidatus Liberibacter resisting chemical through catharanthus roseus

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Embodiment Construction

[0017] The present invention will be described in detail below in conjunction with specific embodiments.

[0018] (1) The phloem bacilli infected with citrus Huanglongbing plants were transmitted to the vinca flowers cultivated by seeds through the haustoria of the dodder connected with the vascular bundles of the host plant. After 2 months, the leaves of the periwinkle showed symptoms of phloem bacillus infection, which was detected by PCR , It was determined that the periwinkle plants were susceptible and kept in an insect-proof greenhouse.

[0019] The PCR detection method is as follows: ① Extraction of DNA of pathogenic bacteria: extraction with Qiagen plant DNA extraction kit (DNeasy Plant Mini Kit, Cat. 69106, Qiagen, MO, USA). Tear out the midrib of the susceptible periwinkle leaves, put about 200mg of the midrib into a 2.0ml centrifuge tube, add AP1 buffer and RNAase, crush it with a homogenizer (Fast Prep24), and incubate at 65°C for 20min During this period, the tes...

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Abstract

The invention relates to a method for screening a Candidatus Liberibacter resisting chemical through catharanthus roseus. The method includes the steps of cultivation of diseased catharanthus roseus plants, cutting regeneration of catharanthus roseus, optimization of cutting regeneration conditions of catharanthus roseus, chemical treatment and screening of the Candidatus Liberibacter resisting chemical. According to the method, the natural host catharanthus roseus for the plant Candidatus Liberibacter is taken as a test material for screening the Candidatus Liberibacter resisting chemical, the screening method is fast, easy and convenient to operate, and the effect of the chemical to be screened on resistance to Candidatus Liberibacter can be evaluated visually and accurately.

Description

technical field [0001] The invention relates to a method for screening antibacterial drugs, in particular to a method for using periwinkle flowers to screen anti-phloem bacillus drugs, and belongs to the technical field of plant protection. Background technique [0002] Phloem bacillus (Ca.Liberibacter) is a pathogen that causes serious diseases such as citrus greening disease and potato zebra flake disease, which has caused huge losses to the production of citrus and potatoes in the world. For example, citrus huanglongbing was first discovered in Florida, USA in 2005. In just 5 years, it has spread to all 34 citrus producing counties, causing economic losses of up to 4.6 billion US dollars; similarly, potato zebra chips The disease is a new disease first discovered in South America in the mid-1990s. In recent years, the disease has spread in the United States, Mexico and New Zealand, posing a serious threat to local potato production. This type of bacteria has not yet been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06A01C1/00
Inventor 张木清
Owner GUANGXI UNIV
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