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Method for large scale fermentation production of Bacillus subtilis antibacterial lipopeptide

A technology of Bacillus subtilis and antibacterial lipopeptide, which is applied in the field of large-scale fermentation and production of Bacillus subtilis antibacterial lipopeptide, which can solve the problems of high production cost, difficulty in batch production, and high price, and achieve low cost, stable product quality, and production short cycle effect

Inactive Publication Date: 2014-04-30
JIANGSU HAIHUA BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The natural content of antimicrobial lipopeptides in organisms is very small, the natural resources are very limited, and the extraction efficiency is very low
However, the production cost by chemical synthesis is very high, the price is expensive, and it is difficult to produce in batches.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0021] A method for large-scale fermentation and production of Bacillus subtilis antimicrobial lipopeptides. Firstly, a seed fermenter with a nominal volume of 2000L is used for strain expansion cultivation.

[0022] Make seed medium: glucose 15g / L, yeast extract 3g / L, tryptone 3g / L, MgSO 4 5g / L, adjust the initial pH value to 6.5, and the fermentation tank liquid coefficient is 65%, with the Bacillus subtilis (Bacillus subtilis) strain fmbR (preservation date: October 31, 2008, preservation number: CICC 10366), inoculated at 5% by volume, cultivated at 37°C for 10 hours, and the ventilation rate during seed cultivation was 1.3m 3 / min, stirring speed 180rpm, tank pressure 0.09-0.11MPa.

[0023] The strains cultured in the 2000L seed fermenter were transferred into a production fermenter with a nominal volume of 20KL according to the volume percentage of 10%, and cultivated at 37°C for 36 hours. Fermentation medium: glucose 30g / L, tryptone 10g / L , beef extract 0.5g / L, MgSO ...

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PUM

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Abstract

The invention relates to a method for large scale fermentation production of a Bacillus subtilis antibacterial lipopeptide. According to the present invention, a microorganism fermentation method is adopted to achieve large-scale industrial production of the antibacterial lipopeptide in a 20KL fermentation tank, wherein the production period is short, the cost is low, the fermentation yield is high, and the extraction yield can be easily increased.

Description

technical field [0001] The invention relates to a method for large-scale fermentation and production of Bacillus subtilis antibacterial lipopeptide, which belongs to the technical field of microbial fermentation. Background technique [0002] In recent years, the problem of food safety in my country has become more and more prominent, and one of the most important reasons is the abuse of food additives. At present, most of the preservatives commonly used in the world food industry are chemically synthesized preservatives. According to the provisions of my country's "Food Additives Use Standard" GB2760, there are only 2 biological additives among the 32 kinds of food preservatives currently allowed to be used, namely Nisin. and actinomycetes natamycin (Natamycin), the rest are chemically synthesized preservatives. Because chemical preservatives are toxic to the human body to varying degrees, and some of them are even carcinogenic and teratogenic, they can cause food poisoning...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12R1/125
Inventor 章金元陈德全石建伟杨晓博
Owner JIANGSU HAIHUA BIOTECH
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