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Method for lavaging hematopoietic stem cells from placenta

A technology for hematopoietic stem cells and placenta, which is applied to blood/immune system cells, animal cells, vertebrate cells, etc., can solve the problems of small number of hematopoietic stem cells, cells are susceptible to bacteria, complicated methods, etc., and achieves less reagents and anti-host response. Small, simple device effect

Active Publication Date: 2014-04-30
SHANDONG QILU STEM CELL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Patent CN01131190.8 discloses a method of obtaining hematopoietic stem cells by shredding and digesting. The method is complicated, the number of hematopoietic stem cells obtained is small, the purity is low, and the cells are easily contaminated with bacteria

Method used

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  • Method for lavaging hematopoietic stem cells from placenta
  • Method for lavaging hematopoietic stem cells from placenta
  • Method for lavaging hematopoietic stem cells from placenta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Method of perfusing hematopoietic stem cells from the placenta

[0049] The placenta came from the obstetrics and gynecology department of the local hospital. First, after obtaining the consent of the pregnant woman or her family to donate the placenta, check all the pregnant women’s examination reports and confirm that there is no virus, syphilis or blood-related viral infection, and then ask the pregnant woman’s family medical history, infection and infection history. After confirming that everything is normal, collect 5 mL of maternal blood, put the placenta and maternal blood into the placenta storage and transportation box, and label and bar code. The temperature in the box during the storage and transportation of the placenta is maintained at 4-10 ℃, and it is transported to the laboratory within 24 hours.

[0050] After arriving in the laboratory, enter the barcode and label into the computer, take out the placenta carefully with tweezers, and gently place...

Embodiment 2

[0054] Example 2 Method for perfusing hematopoietic stem cells from placenta

[0055] The placenta came from the obstetrics and gynecology department of the local hospital. First, after obtaining the consent of the pregnant woman or her family to donate the placenta, check all the pregnant women’s examination reports and confirm that there is no virus, syphilis or blood-related viral infection, and then ask the pregnant woman’s family medical history, infection and infection history. After confirming that everything is normal, collect 5 mL of maternal blood, put the placenta and maternal blood into the placenta storage and transportation box, and label and bar code. The temperature in the box during the storage and transportation of the placenta is maintained at 4-10 ℃, and it is transported to the laboratory within 24 hours.

[0056] Refer to the method in Example 1 to perform the initial inspection of the placenta, pre-sterilization of the placenta, and detection of the placen...

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Abstract

The invention provides a method for lavaging hematopoietic stem cells from a placenta. The method comprises the following six steps: collecting the placenta, initially detecting the placenta, disinfecting the placenta in advance, detecting the placenta and maternal blood, lavaging the placenta hematopoietic stem cells, and concentrating and purifying the hematopoietic stem cells. The method and devices used are simple and convenient to operate; the eluted hematopoietic stem cells are low in damage, large in amount, high in concentration and strong in activity, can be applied for autologous or xenogenous hematopoietic stem cell transplantation, and are helpful to repair damaged tissues.

Description

technical field [0001] The invention relates to a method for perfusing hematopoietic stem cells from placenta. Background technique [0002] At present, hematopoietic stem cell transplantation is mainly adopted in the treatment of hematological diseases in the world. According to the source of cells, it can be divided into three categories: bone marrow hematopoietic stem cell transplantation (BMT), mobilized peripheral blood stem cell transplantation (MPST) and umbilical cord blood stem cell transplantation. (UCBT). The first two sources of stem cells are rich, and the number of nucleated cells can generally reach 5-10×10 8 / Kg, CD34+ cells (a surface marker of hematopoietic stem / progenitor cells) can reach 1-5×10 6 / Kg, but because the HLA must be strictly matched between the donor and the recipient, the success of the transplantation can be guaranteed. Otherwise, the hematopoietic stem cells of the donor are not easy to engraft in the patient's body, and even if they sur...

Claims

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Application Information

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IPC IPC(8): C12N5/0789
Inventor 戴晓宇李栋
Owner SHANDONG QILU STEM CELL ENG
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