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A kind of preparation method of nystatin

A nystatin and the obtained technology, which are applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of low yield of impurities, difficulty in controlling large-scale industrial production, etc., and achieve high-yield production. Effect

Active Publication Date: 2016-04-27
BEIJING CHENGYI INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The method described above can produce crystalline nystatin, but it is controversial due to the presence of impurities and low yield, and it is difficult to control and carry out large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The specific steps are:

[0034] (1) adding n-propanol and / or isopropanol in the fermentation broth of Streptomyces knowersei, so that its volume percentage in the mixed solution is 65%;

[0035] (2) adjusting the pH value of the mixed solution obtained in step (1) to 4.5, then 7.0; filtering to remove undissolved substances, evaporating to remove the organic solvent, and obtaining nystatin precipitation;

[0036] (3) The nystatin precipitation obtained in step (2) is slurried in 30v / v% isopropanol aqueous solution containing 2.2% sodium hexametaphosphate in g / ml, and the gained slurry is warmed up to 55°C, and then Cool to 6°C and recover nystatin from the slurry;

[0037] (4) The nystatin recovered in step (3) is dissolved in the methanol-calcium chloride solution whose mass volume percentage of calcium chloride is 2%, and 1 / 2 volume of water is added to adjust the pH value of the solution to 6.0 , filtered to remove undissolved matter, adjusted the pH of the result...

Embodiment 2

[0039] The specific steps are:

[0040] (1) adding n-propanol and / or isopropanol in the fermentation broth of Streptomyces knowersei so that its volume percentage in the mixed solution is 55%;

[0041] (2) adjusting the pH value of the mixed solution obtained in step (1) to 5.5, then 7.2; filtering to remove undissolved substances, evaporating to remove the organic solvent, and obtaining nystatin precipitation;

[0042] (3) The nystatin precipitation obtained in step (2) is slurried in 20v / v% isopropanol aqueous solution containing 2.0% sodium hexametaphosphate in g / ml, and the gained slurry is warmed up to 58°C, and then Cool to 8°C and recover nystatin from the slurry;

[0043] (4) The nystatin recovered in step (3) is dissolved in the methanol-calcium chloride solution whose mass volume percentage of calcium chloride is 3%, and 1 / 2 volume of water is added to adjust the pH value of the solution to 6.1 , filtered to remove undissolved matter, adjusted the pH of the resulti...

Embodiment 3

[0045] The specific steps are:

[0046] (1) adding n-propanol and / or isopropanol in the fermentation broth of Streptomyces knowersei so that its volume percentage in the mixed solution is 70%;

[0047] (2) adjusting the pH value of the mixed solution obtained in step (1) to 6.0, then to 7.5; filtering to remove undissolved substances, evaporating to remove the organic solvent, and obtaining nystatin precipitation;

[0048] (3) The nystatin precipitation obtained in step (2) is slurried in 40v / v% isopropanol aqueous solution containing 3.0% sodium hexametaphosphate in g / ml, and the gained slurry is warmed up to 58°C, and then Cool to 8°C and recover nystatin from the slurry;

[0049] (4) The nystatin recovered in step (3) is dissolved in the methanol-calcium chloride solution whose mass volume percentage of calcium chloride is 3%, and 1 / 2 volume of water is added to adjust the pH value of the solution to 5.9 , filtered to remove undissolved matter, adjusted the pH of the result...

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Abstract

The invention discloses a preparation method of nystatin. The preparation method comprises steps of extracting nystatin from a streptomyces noursei fermentation broth through a water-soluble organic solvent and further purifying and crystallizing the nystatin. The preparation method disclosed by the invention, through special extraction process as well as purification and crystallization process, can prepare high-yield and high-purity nystatin crystal, and is applicable to industrial production on a large scale.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to a preparation method of nystatin bulk medicine. Background technique [0002] Nystatin is an antifungal antibiotic isolated from the fermentation of Streptomycesnoursei discovered by Hazen and Brown in 1950. It began to be used clinically in 1956. The prevention and treatment of candidiasis caused by antibiotics has good effect. [0003] A method for the preparation of nystatin from Streptomyces knowersii is described in co-pending application serial no. 208,978, filed January 12, 1951, by Hazen and Brown. Crude nystatin concentrate can be prepared by extracting the mycelial mat with several fractions of methanol, followed by fractional precipitation with ethyl acetate, washing the precipitate with 0.85% NaCl solution, dissolving the precipitate in methanol and fractional precipitation with diethyl ether to treat the methanol solution . Active crystalline material...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/08C07H1/08
Inventor 程雪翔
Owner BEIJING CHENGYI INVESTMENT CO LTD
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