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Application of 3'-deoxyadenosine in preparation of immune adjuvant for broad-spectrum vaccine synergism

A deoxyadenosine and immune adjuvant technology, applied in the biological field, can solve the problems of ineffective attenuated strains, weak immunogenicity, and difficulties in the development of attenuated strains

Active Publication Date: 2016-09-28
FUDAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002] Vaccines are an effective means to control many severe infectious diseases. Most of the currently used vaccines are attenuated strains of related pathogens. Obvious and there is a risk of virus reverse mutation
Although the newly developed recombinant nucleic acid vaccines and synthetic peptide vaccines have high purity and strong specificity, they are small in size and weak in immunogenicity, making it difficult to induce an effective immune response in the body. Adjuvants are needed to enable them to produce Antibodies or cellular immune response sufficient to fight infection

Method used

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  • Application of 3'-deoxyadenosine in preparation of immune adjuvant for broad-spectrum vaccine synergism
  • Application of 3'-deoxyadenosine in preparation of immune adjuvant for broad-spectrum vaccine synergism
  • Application of 3'-deoxyadenosine in preparation of immune adjuvant for broad-spectrum vaccine synergism

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Experimental program
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Embodiment Construction

[0023] 1. Evaluation of the effect of 3´-deoxyadenosine on the efficacy of rabies virus vaccine.

[0024] 1. ELISA method (enzyme-linked immunosorbent assay) to detect the concentration of anti-rabies virus antibody and related cytokines in mice after immunization

[0025] The experimental animals were divided into experimental group and control group. Taking BALB / c mice as an example, experimental group 1 was intraperitoneally injected with 0.25ml human rabies vaccine (Vero cells), and experimental group 2 was intraperitoneally injected with 50 micrograms of 3´-deoxyadenosine (dissolved in 10 microliters of DMSO) and 0.25ml Rabies vaccine (Vero cells) was used for humans, and the control group was injected with the same dose of normal saline. Mice in each group were immunized once on the 0th day, the seventh day and the fourteenth day, and the injection site and dosage were the same. Blood was collected from the tail vein of mice on days 0 (before the first injection), 7 (b...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to an immune adjuvant and a preparation method thereof. Provided is a natural immune potentiating molecule 3'-deoxyadenosine, or a mixture or covalent conjugate composed of 3'-deoxyadenosine and MHC epitope peptide to enhance the synergistic effect on vaccines and antigens. The immune adjuvant of the present invention can effectively improve the immune effects of various vaccines (antigens) such as rabies vaccine, hepatitis B vaccine, tuberculosis vaccine and various antigens, and significantly improve the protective antibody titer and the number and effector immune cells in animals. function while significantly accelerating the time to antibody and effector cell production. The preparation method of the immune adjuvant of the invention is easy to implement, can be used to develop high-efficiency and rapid-increasing vaccines, has excellent application prospects in the medical field, and will generate huge social and economic benefits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of a 3'-deoxyadenosine and its composition in the preparation of a broad-spectrum vaccine (or antigen) synergistic immune adjuvant. Background technique [0002] Vaccines are an effective means to control many severe infectious diseases. Most of the currently used vaccines are attenuated strains of related pathogens. There is a clear and present danger of the virus producing back mutations. Although the newly developed recombinant nucleic acid vaccines and synthetic peptide vaccines have high purity and strong specificity, they are small in size and weak in immunogenicity, making it difficult to induce an effective immune response in the body. Adjuvants are needed to enable them to produce Antibodies, or a cellular immune response, sufficient to fight infection. [0003] The innate immune system responds quickly to pathogens, and is usually able to recogn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61P37/04
Inventor 朱乃硕杨艳刘蕊白豆蕫越
Owner FUDAN UNIV
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