Isolated working heart dual-channel perfusion experiment method and device for large animals

Abstract

The invention relates to an isolated working heart dual-channel perfusion experiment method and device for large animals, in particular to isolated working heart dual-channel perfusion experiment method and device for large experimental animals such as pigs or large dogs. The method and device has the advantages that consumption of oxygen-rich anticoagulant blood is saved, heart performance is tested more comprehensively and required equipment is simple. The method is characterized by including: stopping an animal heart, subjecting bilateral coronary, pulmonary artery, pulmonary vein and artery to catheterization, and perfusing a coronary system with the oxygen-rich anticoagulant blood by means of bilateral coronary catheterization; recovering blood pumped out of the pulmonary artery from a right ventricle, testing with an experimental device, oxygenating, and perfusing the coronary with the oxygenated oxygen-rich anticoagulant blood to form an anticoagulant blood closed loop; perfusing with constant temperature physiological solution by a catheter in the pulmonary vein, and recovering the physiological solution through a catheter in the artery to form a physiological solution closed loop; reviving the heart by electrical stimulation.

Description

technical field [0001] The invention belongs to the technical field of basic medical experiments, and in particular relates to a double-channel perfusion experiment method and equipment for isolated working hearts of large experimental animals such as pigs or large dogs. Background technique [0002] The isolated working heart experiment is a common technique in cardiovascular experiments. It can eliminate the influence of the nervous, respiratory, endocrine and other systems, and observe the response of the heart itself to various environmental factors or drug factors. It is the best experimental method at the organ level in circulatory experiments. . [0003] In the classic working heart experiment method, after the heart pumps out the oxygen-containing physiological solution, due to the afterload pressure, part of the physiological solution is infused into the coronary circulation, and this part of the physiological solution maintains the blood supply and oxygen supply of...

AI Technical Summary

Problems solved by technology

According to theoretical calculations, when using the in vitro working heart experiment method, the amount of oxygen-enriched anticoagulant blood required is about 5L / min or more, and the exchange area of ​​the artificial lung system used is so large that it cannot be realized under laboratory conditions.

This makes it difficult to realize large animal in vitro working experiments

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