Detection method of L-erythrothioneine
A technology for ergothioneine and a detection method, applied in the field of chemical analysis, can solve problems such as being difficult to achieve effective separation of a target peak and an impurity peak, affecting the accurate quantitative detection of ergothioneine, and many pretreatment procedures, and achieving low cost and improved use. Longevity and cost reduction effect
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Embodiment 1
[0040] The pretreatment method of the mycelium: the fermentation broth is first filtered by suction to collect the mycelium, and then add the same volume of pure water as the fermentation broth to suspend to make a mycelium suspension. The mycelium suspension was leached once by a 600rpm magnetic stirrer, the leaching temperature was 90°C, and the leaching time was 60min. After leaching, centrifuge for 10 minutes by ultrafiltration, the pore size of the ultrafiltration membrane is 3kDa, and the centrifugation condition is 12840×g. The permeate is the ergothioneine sample to be tested.
Embodiment 2
[0042] The pretreatment method of the mycelium: the fermentation broth is first filtered by suction to collect the mycelium, and then add the same volume of pure water as the fermentation broth to suspend to make a mycelium suspension. The mycelium suspension is directly extracted without stirring, the extraction temperature is 100° C., and the extraction time is 10 minutes. After leaching, centrifuge for 10 minutes by ultrafiltration, the pore size of the ultrafiltration membrane is 3kDa, and the centrifugation condition is 12840×g. The permeate is the ergothioneine sample to be tested.
Embodiment 3
[0044] The pretreatment method of the mycelium: the fermentation broth is first filtered by suction, the mycelium is collected, and one-third of the volume of the fermentation broth is added into pure water to suspend to make a mycelium suspension. The mycelium suspension was leached in a water bath, the leaching temperature was 50°C, the leaching time was 30min, and the stirring speed was 500rpm. After leaching, the solid-liquid separation is realized through suction filtration, and the obtained mycelium is subjected to suction filtration again, and the method is the same as above, and the leaching process is repeated three times in this way, and the total extraction time is 90min, and the three extractions are mixed uniformly to obtain A mixed extract with the same volume as the fermentation broth. After leaching and mixing, ultrafiltration and centrifugation for 10 minutes, the pore size of the ultrafiltration membrane is 3kDa, and the centrifugation condition is 12840×g. ...
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