Preparation method of Sinonovacula constricta polypeptide with antioxidation function and application thereof

Abstract

The invention provides a preparation method of Sinonovacula constricta enzymatic hydrolysis polypeptide, and the invention is characterized in that the method: shelling Sinonovacula constricta, cleaning and rubbing, adding water according to a mass ratio which is 1:1-4 between the material and the water for homogenate, and adjusting the pH value to 7-8; adding protease for enzymatic hydrolysis, and the addition of the protease is 0.2-0.3 m% of Sinonovacula constricta, and the enzymatic hydrolysis temperature is 60-70 DEG C, and the hydrolysis time is 2-6 hours; after enzymatic hydrolysis, killing enzyme, fetching an upper clear liquid by centrifugation, and obtaining an enzymatic hydrolysis liquid; obtaining the required Sinonovacula constricta enzymatic hydrolysis polypeptide by ultrafiltration, G-25 gel chromatographic column chromatography and reversed-phase high performance liquid chromatography elution of the enzymatic hydrolysis liquid. The invention has the advantages of simple preparation technology, and the prepared Sinonovacula constricta enzymatic hydrolysis polypeptide has the advantages of high sensitivity, good stability and little side-effect, good hydroxyl radical clearance, DPPH free radical clearance and redox performance with substantial antioxidation effect; the Sinonovacula constricta enzymatic hydrolysis polypeptide can be used for external antioxidation, and has an important meaning for further research and development of functional foodstuff and medicament based on Sinonovacula constricta enzymatic hydrolysis polypeptide, and improvement of economic value of Sinonovacula constricta.

Description

technical field [0001] The invention belongs to marine functional foods, in particular to an anti-oxidative marine functional food and a preparation method thereof. Background technique [0002] Marine shellfish is rich in resources and rich in many physiologically active substances, so it has been widely studied and applied gradually. Constricted razor clam (Sinonovacula constricta), commonly known as razor clam, belongs to Petalbranchia, Heterodontia, Pleurophyllidae, Lighthouse Clamidae, and Constricted razor clam. It is distributed in the coastal areas of my country and has a large output. It is one of the four major cultured shellfish in my country[ An Xianhui, Li Liantai. Research status and development prospect of razor clam [J]. Scientific Fish Farming, 2005 (1): 4-6.] The meat of razor clam is delicious and nutritious, rich in various essential amino acids and unsaturated fatty acids required by human life activities Nutrients [Li Taiwu, Lin Ye, Su Xiurong, Multiva...

AI Technical Summary

Problems solved by technology

For razor clam constriction, there are few researches on anti-oxidation, especially the research on anti-oxidation of peptides extracted by enzymatic hydrolysis technology has not been reported.

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