Method for biodegrading chlorophenols

A technology of biodegradation and chlorophenols, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve a rapid response effect

Inactive Publication Date: 2014-04-23
LISHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Leucobacterium laccase for the degradation of chlorophenols

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Strains

[0018] The strains of Armillariella tabescens were purchased from China General Microorganism Culture Collection and Management Center (CGMCC No.5.0013, isolation number AS3.2624), and cultured on PDA slant at 27°C.

[0019] 2. Medium

[0020] Comprehensive PDA solid medium: glucose 20g, agar powder 18g, potato juice 200g, MgSO 4 ·7H 2 O1.5g, KH 2 PO 4 3g, VB 1 0.05g, make up to 1L with distilled water;

[0021] Liquid seed medium: glucose 20g, peptone 15g, KH 2 PO 4 5g, MgSO 4 ·7H 2 O1g, (NH 4 ) 2 SO 4 5g, 20g corn flour, make up to 1L with distilled water, pH6.0; the composition of the liquid fermentation medium is the same as that of the liquid seed medium.

[0022] 3. Fermentation culture

[0023] 3.1 Strain culture

[0024] Inoculate on slant or plate culture medium under sterile conditions, and incubate at 26°C in the dark for 7 days.

[0025] 3.2 Liquid seeds

[0026] Under sterile conditions, use a puncher (diameter 0.5cm) to measur...

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PUM

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Abstract

The invention provides a method for biodegrading chlorophenols. The method comprises the following step: by using armillariella tabescens laccase obtained by fermentation of armillariella tabescens as a biological catalyst, degrading the chlorophenols in waste water under the conditions that the temperature is 50-60 DEG C and the pH is 3.6-4.5, wherein the chlorophenols is one or a mixture of more than two of 2,4-dichlorophenol, 2-chlorophenol and 4-chlorophenol. The chlorophenols are organic matters which are most harmful to the environment. According to the method, the chlorophenols can be effectively degraded. By virtue of quick reaction and action for 14h, the degradation rate of the chlorophenols can reach 50-90 percent.

Description

(1) Technical field [0001] The invention relates to a method for biodegrading chlorophenol compounds. (2) Background technology [0002] With the rapid development of industry, the economy has developed, but the environmental pollution has become more and more serious. Chlorophenols are a class of refractory compounds that have been widely used in the production of wood preservatives, pesticides and herbicides for a long time; among them, 2,4-dichlorophenol (DCP) is a toxic and refractory pollutant It is widely found in the production wastewater of pesticides, fuels and plasticizers. The United States, China and other countries have included it in the "blacklist" of harmful substances or priority pollutants in water. Therefore, how to eliminate the pollution of chlorophenol compounds in the environment has become a subject of extensive attention. At present, common methods to control chlorophenol pollution include ultrafiltration, chemical sedimentation, activated sludge,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02C12N9/02A62D101/22A62D101/28C12R1/645
Inventor 叶选怡凌庆枝杨丽红钟仙龙何军邀
Owner LISHUI UNIV
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