A kind of breeding method of in vitro induction autotetraploid broccoli

Abstract

The invention belongs to the field of crop breeding and discloses a breeding method for in-vitro induction of autotetraploid brassica oleracea l. var. italica plenck. The breeding method comprises the following steps: reserving a 0.4-0.6-cm seedling of a hypocotyl as an explant in the cotyledon period of the brassica oleracea l. var. italica plenck, inoculating the explant to a bilayer differentiation culture medium containing colchicine and performing induction culture for 45-50h, and directly inducing to grow a tetraploid bud, thus further obtaining the autotetraploid brassica oleracea l. var. italica plenck rapidly and stably. According to the breeding method disclosed by the invention, an asexual polyploidization breeding way is adopted for the first time, the bilayer induction based on the liquid culture medium (with addition of colchicine) and the solid culture medium (without addition of colchicine) is adopted, and the 0.4-0.6-cm seedling of the hypocotyl reserved in the cotyledon period of the brassica oleracea l. var. italica plenck is processed in a combinational way to obtain a new germplasm of the tetraploid brassica oleracea l. var. italica plenck. The breeding method has the advantages of high induction rate, fewer chimera, low death rate and low contamination rate, a large quantity of tetraploid plants can be obtained by performing meristematic proliferation on the obtained tetraploid material, and thus, the induction time is greatly shortened.

Description

technical field [0001] The invention belongs to the field of crop breeding, and relates to a breeding method for in vitro induction of autotetraploid broccoli, which is specially used for the breeding of tetraploid broccoli. Background technique [0002] Broccoli (Brassica oleracea Lvaritalica Plenck), also known as broccoli, cauliflower, and green cauliflower, belongs to the genus Brassica in the family Brassicaceae. It is a variety of cabbage in the family Brassicaceae. The natural species and all cultivated species are diploid, that is, 2n=2x=18. Broccoli is a crop suitable for chromosome doubling improvement (Ji Dandan, Zhao Xiaoming. 2007. The anticancer effect of broccoli and the research prospect of medicinal polyploid breeding. Chinese Vegetables, (8): 39-41.). At present, many high-quality diploid broccoli varieties are bred by using some breeding methods such as dominant breeding, conventional breeding, and tissue culture in my country, but the cultivation of poly...

AI Technical Summary

Problems solved by technology

At present, in the existing technology at home and abroad, there is no double-layer induction of liquid medium (adding colchicine) and solid medium (without adding colchicine) combined with the treatment of seedlings with hypocotyls of 0.4-0.6 cm at the cotyledon stage. Clonal Polyploidy Breeding Technology of Ploidy Broccoli to Obtain New Tetraploid

Images