Salviae miltiorrhizae artificial cultivation method
A technology of artificial cultivation and salvia miltiorrhiza, applied in cultivation, botany equipment and methods, seed and rhizome treatment, etc., can solve the problems of quality and yield decline, unstable content of active ingredients, low germination rate of salvia miltiorrhiza seeds, etc., to promote germination Effect
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[0029] The present invention will be described in detail below in conjunction with the accompanying drawings.
[0030] In the previous work of the present invention, an endophytic fungus TR21 producing tanshinone ⅡA was isolated, and three strains of endophytic fungi capable of high-yield tanshinone ⅡA were induced by mutagenesis using TR21 as the starting strain (see Document 1). The purpose of this study is to observe the effects of these four strains on the growth of Salvia miltiorrhiza, and solve the problems of artificially cultivated salvia miltiorrhiza from the perspective of endogenous sources.
[0031] Literature (1) Caixia Ma, Dongliang Jiang, Xiying Wei. Mutation breeding of Emericella foeniculicola TR21 for improved production of tanshinone IIA. Process Biochemistry, 2011,46(10):2059~2063.
[0032] 1 Materials and methods
[0033] 1.1 Materials
[0034] 1.1.1 Medium
[0035] Potato dextrose agar medium (PDA): 200g potato, 20g glucose, 18g agar, 1000mL distilled ...
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