Serum/plasmanon miRNA marker for diagnosing non-small cell lung cancer at early stage and application thereof
A non-small cell lung cancer, early diagnosis technology, applied in the fields of genetic engineering and oncology, can solve the problems of loss of treatment time, increase of lung cancer mortality rate, lung cancer screening and diagnosis, etc., to achieve rapid direction, improve sensitivity and specificity, Easy to detect effects
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[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0021] The use, method and kit of the present invention can be effectively used in the selection of tumor treatment plan and / or prognosis assessment, thereby providing a novel tumor diagnostic agent and / or therapeutic agent, which has a certain clinical application prospect. The specific implementation steps are as follows:
[0022] (1) Reaction system and conditions of Q-PCR
[0023]
[0024]
[0025] (2) Configuration of Q-PCR
[0026]
[0027] (3) Amplification ...
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