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Identification method and equipment for sensibility of bacteria on antibiotic

An identification method and antibiotic technology, applied in the field of microorganisms, can solve complex problems and achieve the effect of shortening the time required for identification

Inactive Publication Date: 2014-04-16
佟雪梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the turbidimetric method is often used to detect the sensitivity of bacteria to antibiotics, which is identified according to the number of bacteria growing in a solution containing antibiotics. However, the process of killing bacteria by antibiotics is relatively complicated, and sometimes bacteria are partially exposed to antibiotics. The bacteria are killed and the turbidity drops, but because some bacteria are induced to produce enzymes, the bacteria start to grow again. Therefore, it takes a long time to detect the changes in the turbidity of the bacteria to know the sensitivity to the drug. sensitivity

Method used

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  • Identification method and equipment for sensibility of bacteria on antibiotic
  • Identification method and equipment for sensibility of bacteria on antibiotic
  • Identification method and equipment for sensibility of bacteria on antibiotic

Examples

Experimental program
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Effect test

Embodiment 1

[0079] Establishment of Escherichia coli susceptibility database to ceftazidime and identification of Escherichia coli susceptibility to ceftazidime.

[0080] (a) Take 60 strains of Escherichia coli known to be sensitive to ceftazidime, 50 strains of Escherichia coli known to be moderately sensitive to ceftazidime, and 30 strains of Escherichia coli known to be resistant to ceftazidime; The ceftazidime-resistant MIC configuration was used to induce antibiotics for the strains, specifically: each strain was prepared with 0.9% normal saline to make 0.5 McFarland units; 0.4ml of ceftazidime with a concentration of 80μg / ml was added to 1.6ml of MH broth, and then Add 1ml of the strain suspension respectively, and incubate at 37°C for 4 hours.

[0081] (b) Centrifuge the culture solution at 4°C to obtain a precipitate, add 1.5ml-45°C cold methanol (inactivation and extraction dual function) and 0.75ml cold pure water, vortex and shake to mix, then add -45°C chloroform 1.5ml Vortex...

Embodiment 2

[0087] Establishment of Penicillin G Sensitivity Database of Staphylococcus aureus and Identification of Staphylococcus aureus Sensitivity to Penicillin G.

[0088] (a) Take 60 strains of Staphylococcus aureus known to be sensitive to penicillin G, 50 strains of Staphylococcus aureus known to be moderately sensitive to penicillin G, and 30 strains of Staphylococcus aureus known to be resistant to penicillin G; as recommended by CLSI ATCC29213 is resistant to penicillin G with the MIC configuration to induce antibiotics for the strains, specifically: each strain is prepared with 0.9% normal saline to make 0.5 McFarland units; respectively take 0.4ml of penicillin G with a concentration of 1.25μg / ml and add 1.6 ml MH broth, and then add 1ml of strain suspension respectively, and incubate at 37°C for 4 hours.

[0089] (b) Centrifuge the culture solution at 4°C to obtain a precipitate, add 1.5ml-45°C cold methanol (inactivation and extraction dual function) and 0.75ml cold pure wa...

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Abstract

The invention relates to the field of microorganisms and particularly relates to an identification method and equipment for sensibility of bacteria on antibiotic. The method comprises the following steps: respectively carrying out antibiotic induction on known sensitive bacteria, intermediate sensitive bacteria and drug-resisting bacteria; extracting intracellular metabolites; carrying out mass spectrometry on the intracellular metabolites to obtain a mass spectrum pattern; converting all information of the mass spectrum pattern into a characteristic peak form and carrying out a clustering analysis on a corresponding metabolite wave peak value to respectively obtain clustering patterns of strains which are sensitive, intermediately sensitive and drug-resisting; removing wrongly-classified strains and storing in a database; treating bacteria to be detected by the same steps and combining the metabolite wave peak values of the bacteria to be detected with the obtained database; and reclassifying to obtain the positions of the bacteria to be detected in the clustering patterns, so as to obtain the antibiotic sensibility of the bacteria to be detected. According to the identification method and equipment for the sensibility of the bacteria on the antibiotic, the time for identifying is shortened.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a method and equipment for identifying the susceptibility of bacteria to antibiotics. Background technique [0002] Drug susceptibility test, referred to as drug susceptibility test (or drug resistance test), is to understand the sensitivity (or tolerance) of pathogenic microorganisms to various antibiotics, so as to guide the rational selection of antibiotics in clinical practice. [0003] At present, the turbidimetric method is often used to detect the sensitivity of bacteria to antibiotics, which is identified according to the number of bacteria growing in a solution containing antibiotics. However, the process of killing bacteria by antibiotics is relatively complicated, and sometimes bacteria are partially exposed to antibiotics. The bacteria are killed and the turbidity drops, but because some bacteria are induced to produce enzymes, the bacteria start to grow again. Therefore, it t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12M1/34
CPCY02A50/30
Inventor 佟雪梅
Owner 佟雪梅
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