Strain for producing aryl sulfatase and application thereof

A technology of arylsulfatase and bacterial strains, which is applied in the field of microorganisms, can solve the problems of high gel strength and low electricity endosmosis agar, and achieve the effect of improving high gel strength

Active Publication Date: 2014-04-09
OCEAN UNIV OF CHINA
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AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the screening of arylsulfatase-producing strains from marine resources in China, and there is no report on the production of high gel strength and low-electricity endosmosis agar by using naturally occurring arylsulfatase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1: Screening and identification of strain Marinomonas sp.FW-1

[0023] Microbial enrichment, primary screening, and secondary screening were performed on seaweed samples from the coastal waters of Qingdao to obtain target bacteria with stable enzyme production, and the strains were identified according to their morphology, physiological and biochemical characteristics, and molecular biology.

[0024] (1) Microbial enrichment culture: take an appropriate amount of seaweed samples collected under aseptic conditions in a sterile beaker, add an appropriate amount of sterile sulfate-free artificial seawater (NaCl20.00g, MgCl 2 ·6H 2 O1.61g, KCl5.00g, CaCl 2 1.06g, K 2 HPO 4 ·3H 2 O0.85g, FeCl 2 4H 2 (00.51g, distilled water 1L, pH=8). Seal it with eight layers of medical gauze, and ferment naturally for 5 days in a cool place at room temperature.

[0025] (2) Primary screening of enzyme-producing microorganisms: After the enriched fermentation broth is filtered throu...

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Abstract

The invention relates to a strain for producing aryl sulfatase and application thereof, and particularly provides a strain with preservation number of CCTCC NO: M 2013613, application of the strain in production of aryl sulfatase, a method for producing the aryl sulfatase by use of the strain and a method for producing agar with high gel strength and low electroosmosis. The screened strain can effectively produce the aryl sulfatase which can be used for carrying out enzymatic hydrolysis on agar powder and specifically removing sulfate radicals of agaropectin polysaccharide, and the yield of the agar with high gel strength and low electroosmosis can be greatly improved, and therefore, the strain has important application value in industrial production.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to a production strain of arylsulfatase and its application. 【Background technique】 [0002] Agar (Agar) is derived from red algae such as Geliflower and Gracilaria. It is a polysaccharide with special coagulation and stability, and is widely used in scientific research fields such as food and chemical industry. Its chemical composition includes two parts: agarose without sulfate and sulfur agar with sulfate. Agarose is the gel-forming component, while sulfur agar is the non-gelling part that commercial agar extraction processes try to remove. The gel strength and electroosmosis of agar gel are closely related to the content of sulfate radicals. Those with high sulfate radical content have low gel strength and high electroosmosis value; otherwise, the gel strength is high and the electroosmosis value is small. Agar with different sulfate content has different uses. Agar c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/16C12P19/04C12R1/01
Inventor 付晓婷王志朋王雪艳林洪许加超高昕
Owner OCEAN UNIV OF CHINA
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