A preparation method of chitosan/hyaluronic acid/gelatin cross-linked composite porous scaffold

Abstract

The invention relates to the technical field of novel materials, and in particular relates to a method for preparing a chitosan / hyaluronic acid / gelatin crosslinked composite porous stent. The method uses chitosan, hyaluronic acid, gelatin and other natural biological materials as raw materials and dialdehyde starch as a crosslinking agent, and combines a lyophilisation method and a particulate leaching method to prepare the chitosan / hyaluronic acid / gelatin crosslinked composite porous stent, which is excellent in mechanical performance and made of a pure natural biological material. The chitosan / hyaluronic acid / gelatin crosslinked composite porous stent prepared by the method has the characteristics of high porosity, high pore strength, large pore diameter and the like, the pore diameter is 100-300 mu m, and the porosity is more than 90 percent. The stent is of an opening pore structure with small pores in large pores, and is suitable for cell implanting on the surface of the composite stent. Compared with a chitosan stent, the crosslinked composite porous stent has a better mechanical performance, proliferation and differentiation of cells on the surface of the composite stent can be more favored, and the compatibility of the composite stent to biological cells is excellent.

Description

technical field [0001] The invention belongs to the technical field of new materials, and in particular relates to a preparation method of a chitosan / hyaluronic acid / gelatin cross-linked composite porous support. Background technique [0002] The emergence of tissue engineering has brought bright prospects for human health and life extension. The use of biomedical materials to prepare artificial substitutes to treat diseases can solve the problem that human organs need to be replaced due to defects or loss of function, but the materials are limited. When people put their energy into artificial tissues or organs, one of the keys is the preparation of three-dimensional porous scaffold materials for tissue engineering using cell culture. [0003] At present, porous scaffold materials for tissue engineering mainly come from some natural polymers and synthetic polymers. Natural polymers mainly include collagen, chitin, chitosan, hyaluronic acid, gelatin, alginate, chondroitin su...

AI Technical Summary

Problems solved by technology

Based on the advantages of convenience, easy access, taking from nature and using in nature, taking advantage of the similarity and compatibility between natural biological materials and human tissues, many researchers have chosen natural biological materials to prepare tissue engineering scaffold materials, but natural biological materials The problem of weak mechanical strength generally exists, which requires people to choose suitable natural biomaterials for blending and compounding, and to select effective cross-linking agents to improve their mechanical properties, and the cross-linking agents must also be non-cytotoxic

Images