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Apricot kernel peel fermentation forage produced by solid state fermentation of multiple strains

A technology of solid-state fermentation and fermented feed, applied in the field of protein feed, to achieve the effects of strong adaptability, fast growth, and easy animal decomposition and utilization

Active Publication Date: 2014-12-17
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on solid-state fermentation of almond bark to produce protein feed using the combination of Aspergillus niger, Saccharomyces cerevisiae and Bacillus subtilis

Method used

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  • Apricot kernel peel fermentation forage produced by solid state fermentation of multiple strains
  • Apricot kernel peel fermentation forage produced by solid state fermentation of multiple strains
  • Apricot kernel peel fermentation forage produced by solid state fermentation of multiple strains

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preparation example Construction

[0051] The composition of the PDA medium (potato dextrose agar medium) is: potato extract 1L, glucose 20g, KH 2 PO 4 3g,MgSO 4 1.5g, vitamin B 1 10mg, agar 15g, pH6.0. The preparation method of the potato extract comprises the following steps: weighing 200g of potatoes, washing, peeling, cutting into small pieces, adding 1000ml of water, boiling for 30min, filtering with gauze to obtain clear liquid, adding water to 1000ml.

[0052] The composition of the nutrient broth medium is: 10g of peptone, 3g of beef extract powder, 5g of sodium chloride, and the final pH is 7.4±0.2.

[0053] Other carriers, feed additives, and raw materials can be purchased from the feed market.

Embodiment 1

[0054] Embodiment 1: the almond bark fermented feed produced with multi-species solid-state fermentation

[0055] 1. Raw materials: 100g of almond skin, 20g of solid-state fermentation medium, 180g of water and 40ml of compound microbial starter.

[0056] 2. Preparation method:

[0057] 1) Preparation of compound microbial starter:

[0058] Seed Medium:

[0059] Aspergillus niger: spore suspension, adjust the concentration to 10 7 a / ml;

[0060] Brewer's yeast: PDA medium, adjusted concentration 10 7 a / ml;

[0061] Bacillus subtilis: nutrient broth liquid medium, adjusted concentration 10 7 a / ml;

[0062] Take 20ml of Aspergillus niger, 10ml of Bacillus subtilis, and 10ml of Saccharomyces cerevisiae from each of the above three mediums to make 40ml of composite microbial starter, and mix well;

[0063] 2) Preparation of solid-state fermentation medium

[0064] Take 20g of bran, 2g of urea, 2g of ammonium sulfate, 0.5g of dipotassium hydrogen phosphate, and 0.1g of mag...

Embodiment 2

[0069] Embodiment 2: the almond bark fermented feed produced with multi-species solid-state fermentation

[0070] 1. Raw materials: 100g of almond skin, 20g of solid-state fermentation medium, 156g of water and 30ml of compound microbial starter.

[0071] 2. Preparation method:

[0072] 1) Preparation of compound microbial starter:

[0073] Seed medium:

[0074] Aspergillus niger: spore suspension, adjust the concentration to 10 7 a / ml;

[0075] Brewer's yeast: PDA medium, adjusted concentration 10 7 a / ml;

[0076] Bacillus subtilis: nutrient broth liquid medium, adjusted concentration 10 7 a / ml;

[0077] Take 10ml of Aspergillus niger, 10ml of Bacillus subtilis, and 10ml of Saccharomyces cerevisiae from each of the above three mediums to make 30ml of composite microbial starter, and mix well;

[0078] 2) Preparation of solid-state fermentation medium

[0079] Take 15g of bran, 1g of urea, 1g of ammonium sulfate, 0.5g of dipotassium hydrogen phosphate, and 0.2g of mag...

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Abstract

The invention relates to an apricot kernel peel fermentation forage produced by solid state fermentation of multiple strains. The raw materials contain the following components: apricot kernel peel, a solid state fermentation medium, water and a compound microbial fermentation agent. The experimental results show that the apricot kernel peel fermentation forage produced by solid state fermentation of multiple strains disclosed by the invention increases the protein content of apricot kernel forage and reduces the content of toxic substance amygdalin. The rumen nylon bag degradation test shows that effective degradation rate of the crude protein and neutral detergent fiber in the fermented forage can be significantly improved.

Description

technical field [0001] The invention relates to the field of processing and utilization of roughage for ruminants in the feed industry, in particular to a protein feed produced by solid-state fermentation of multi-strain almond bark. Background technique [0002] Almond bark is the reddish brown or dark yellow seed coat of almonds. Almond bark contains 11.4%-13.1% crude protein and about 45% carbohydrates, which has the potential to become a new type of feed. In the industry, the almond skin is removed from the almond by the hot blanching method, and the peeled almond skin is discarded as waste, which causes a lot of waste and also causes environmental pollution. If the almond skin is directly fed to livestock as feed, the feeding effect will be poor because of the low absorbable nutritional value in the almond skin. [0003] In addition, almond bark also contains an important biologically active substance - amygdalin, which will be decomposed into toxic substance hydrocya...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
CPCY02P60/87
Inventor 屠焰刁其玉张乃锋刘策马涛
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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