Application of proteins of Rv3872, Rv0164 and/or Rv1926c in developing and/or designing product with functions of identification, diagnosis, auxiliary diagnosis, screening and/or auxiliary screening of active tuberculosis

Abstract

The invention provides application of proteins of Rv3872, Rv0164 and / or Rv1926c in developing and / or designing a product with functions of identification, diagnosis, auxiliary diagnosis, screening and / or auxiliary screening of the active tuberculosis. The invention further provides a protein chip prepared by the proteins of Rv3872, Rv0164 and / or Rv1926c. According to the invention, the protein chip can be used for detecting the levels of IgG antibodies corresponding to three protein antigens in the blood serum of a patient with active tuberculosis and those in the blood serum of a normal person, conjointly analyzing the testing results of antibodies corresponding to the three groups of proteins and judging whether people to be tested have active tuberculosis or not. Test results show that the specificity and the sensitivity of the optimal operating point of the protein chip for auxiliary diagnosis of the active tuberculosis are 91.1% and 82.4% respectively which are both above the diagnosis indexes of the active tuberculosis in prior art.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the use of mycobacterium tuberculosis protein in preparing products for diagnosing and / or assisting in diagnosing diseases. Background technique [0002] For centuries, tuberculosis has continued to be a global public health problem that cannot be ignored. At present, one-third of the world's population is carrying Mycobacterium tuberculosis. In 2010 alone, there were 8.8 million new tuberculosis cases and 1.45 million deaths. On average, one person died of tuberculosis in less than 22 seconds. Tuberculosis is the highest infectious disease The number one death toll. my country is one of the 22 countries with a high burden of tuberculosis in the world. The number of tuberculosis patients ranks second in the world, and the number of infected people exceeds 500 million. In 2010 alone, there were 900,000 to 1.2 million new cases, accounting for the total number of new cases in...

AI Technical Summary

Problems solved by technology

However, the antigens currently targeted for serological diagnosis of Mycobacterium tuberculosis, such as antigen 5, 38KD antigen, 30 / 31KD antigen, and antigen 60, all have problems such as low positive detection rate and cross-immunity with other mycobacteria. It has certain value in monitoring, prompting recurrence, judging prognosis and general screening of high-risk groups, but it still cannot be used for the diagnosis of active pulmonary tuberculosis

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