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A technology of armillaria and concentrated solution, which is applied in the direction of fungi, etc., which can solve the problems of long cultivation period, uncertain adenosine content, unclear medium composition, etc., and achieve the effect of shortening the production period and facilitating production operations
Active Publication Date: 2014-03-26
辽宁新高制药有限公司
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Problems solved by technology
However, due to the unclear composition of the culture medium of the Armillaria concentrated solution in the statutory standards, the existing preparation metho
Method used
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Embodiment 1
[0011] 1. Inoculate the Armillaria strain on the sterile medium and culture it at 27°C±1°C for 3 days to obtain the mycelium for use;
[0012] 2. Preparation of sterile culture solution: take 3 parts of glucose, 3 parts of peptone, 1 part of yeast extract, KH 2 PO 4 0.1 parts, MgSO 4 0.5 parts, NaCl0.003 parts, vitamin B10.05 parts, add 100 parts of purified water and mix well;
[0013] 3. Fermentation: inoculate the mycelia into the liquid culture medium, ferment for 3 days at 27°C±1°C, rotation speed 100rpm, ventilation rate 100L / h, pH value about 5.0; concentration: collect the fermentation liquid, filter the fermentation liquid Then concentrate under reduced pressure to a density of 1.032 (20°C).
Embodiment 2
[0015] 1. Inoculate Armillaria strains on sterile medium, and culture them at 27°C±1°C for 4 days to obtain mycelia for use;
[0016] 2. Preparation of sterile culture solution: take 5 parts of glucose, 1 part of peptone, 3 parts of yeast extract, KH 2 PO 4 0.5 parts, MgSO 4 0.1 part, 0.001 part of NaCl, 10.1 part of vitamin B, add 150 parts of purified water and mix well.
[0017] 3. Fermentation: inoculate the mycelia obtained in step 1 into the liquid culture solution prepared in step 2, and ferment at 27°C ± 1°C, rotation speed 500rpm, ventilation rate 150L / h, and pH value 5.0. sky;
[0018] 4. Concentration: collect the fermented liquid, filter the fermented liquid and concentrate under reduced pressure to a density of 1.030-1.035 (20°C).
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Abstract
A preparation method of armillaria concentrate comprises the following steps: inoculating a bacterial strain onto a sterile culture medium, and culturing the bacterial strain for 3-4 days at 27 degrees plus or minus 1 degree to obtain mycelium; in terms of parts by weight, taking 3-5 parts of glucose, 1-3 parts of peptone, 1-3 parts of yeast extract, 0.1-0.5 part of KH2PO4, 0.1-0.5 part of MgSO4, 0.001-0.003 part of NaCl and 0.05-0.1 part of vitamin B1, adding 100-150 parts of purified water, and uniformly mixing to obtain a culture solution; inoculating the mycelium into the culture solution, and fermenting for 3-5 days under the conditions that the temperature is 27 degrees plus or minus 1 degree, the rotating speed is 100-500 rpm, the ventilation amount is 100-150 L/h, and the pH value is 5.0; collecting fermentation liquid, filtering the fermentation liquid, and concentrating the fermentation liquid at reduced pressure till the density (at 20 DEG C) is 1.030-1.035 to obtain the armillaria concentrate. The preparation method of the armillaria concentrate has the advantages as follows: the composition of the culture medium is clarified, so that production operation is facilitated; the production cycle is shortened; the fermentation liquid is relatively high in yield; the adenosine content of the fermentation liquid is higher than that in the original production technology.
Description
Technical field: [0001] The invention belongs to the field of pharmacy, and in particular relates to a method for preparing a concentrated liquid of Armillaria armillaria, a raw material medicine of traditional Chinese medicine. Background technique: [0002] Armillaria mellea (Vahl ex Fr.) Quel is a medicinal and edible fungus belonging to Trichol omataceae (Trichol omataceae) Armillaria (Fr.) Staude. ) symbiotic famous medicinal fungi. After many years of research at home and abroad, various active ingredients such as Armillaria polysaccharides, sesquiterpenoids and purines have been isolated. Armillaria fruiting bodies have hypnotic, sedative, anti-vertigo, improved heart and brain blood circulation and strong brain protection, can treat a variety of diseases, and can improve the immune function of the body. Armillaria Armillaria is one of the nourishing and health-care traditional Chinese medicinal materials in my country, and is favored by scholars at home and abroad....
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Application Information
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