A kind of preparation method of cow's milk mixed type infant formula milk powder
A formula and mixed technology for infants and young children, applied in dairy products, milk preparations, applications, etc., can solve the problem of infants not being able to get breastfeeding, and achieve the effects of improving immunity and resistance, avoiding diarrhea, and promoting growth and development.
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Embodiment 1
[0043] Embodiment 1: the modification of cow's milk casein
[0044] (1) Extraction and activity determination of rennet enzyme
[0045] Calf abomasum→cleaning→fine cutting→extraction (three times)→filtering→dialysis→drying→Renminase rennetase activity determination, please refer to the product manual of Wuxi Enzyme Preparation Factory.
[0046] (2) Separation of cow milk casein
[0047] Centrifuge the fresh milk to remove fat, adjust the pH of the milk to 4.3 with hydrochloric acid, and collect the precipitate, which is casein.
[0048] (3) Human emulsification modification and optimization experiment of casein
[0049] Dissolve the casein precipitate collected in the previous step.
[0050] Casein solution → rennet enzyme action → enzyme inactivation → centrifugation, the supernatant is the modified casein, detected by SDS-PAGE gel electrophoresis, it is found that compared with the original casein, the position of αs-casein The electrophoresis bands weakened a lot, but t...
Embodiment 2
[0052] Embodiment 2: Separation and purification of goat milk β-casein
[0053] (1) Fat removal
[0054] Select fresh goat milk sample (10mL) and centrifuge at 10400g for 30min at 4°C. The sample is divided into three layers, wherein the milk fat part is in the upper layer, the whey part is in the middle layer, and the milk particle part is in the bottom layer. Avoid the upper lipid layer and absorb the whey part. spare.
[0055] (2) Separation of β-casein
[0056] Adjust the whey pH to 4.6, reach the isoelectric point of casein, centrifuge at 40000g for 30min at 4°C, collect the precipitate, weigh 100mg of casein precipitate and dissolve in 50mL of equilibrium buffer (20mmol / L Tris, 3.3mol / L Tris urea), 1M NaOH to adjust the pH to 9.5. The samples were stirred intermittently in a water bath at 20°C for 0.5-1h. After fully dissolving, centrifuge at 10,000 g for 30 min at 4°C, filter the supernatant with a 0.45 μm filter membrane, and pass through a DEAE-Sepharose Fast Flow...
Embodiment 3
[0058] Embodiment 3: Addition of demineralized whey powder
[0059] Simulating the ratio of casein to whey protein in human milk, artificially added desalted whey powder, so that the ratio of casein to whey protein in formula milk powder was 40:60.
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