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Production method of fish cartilage extract for preventing and treating osteoarthritis

A technology for osteoarthritis and production methods, applied in bone diseases, food science, drug combination, etc., can solve the problems of excessive molecular weight and low clinical bioavailability of chondroitin sulfate

Inactive Publication Date: 2014-03-12
QINGDAO BETTER BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the products obtained from the above-mentioned patents have not solved the problem that the molecular weight of chondroitin sulfate is too large and the clinical bioavailability is low.

Method used

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  • Production method of fish cartilage extract for preventing and treating osteoarthritis
  • Production method of fish cartilage extract for preventing and treating osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Wash the salmon cartilage, soak it in water for 2 hours, put it in a cooking pot and cook it at 100°C for 30 minutes, remove the fish meat and oil, wash it with water, chop it into pieces with a size of 1cm×1cm, and set aside; choose the processed Cartilage 500g, add water according to the ratio of material to liquid 1:2, adjust pH=7.0 with sodium hydroxide or hydrochloric acid, add papain 5g / kg, neutral protease 5g / kg and chondroitinase 5g / kg. Control the temperature at 45°C for enzymatic hydrolysis for 5 hours, raise the temperature of the obtained enzymatic hydrolyzate to 80°C for heat treatment for 15 minutes, then lower the temperature to 70°C, centrifuge for 40 minutes at a speed of 2000 r / min, and add an appropriate amount of filter aid kaolin to aid in filtration The obtained supernatant was intercepted by an ultrafiltration membrane with a molecular weight of 5000, and the membrane permeate was collected and spray-dried to obtain a cartilage extract.

[0033] T...

Embodiment 2

[0035] Wash the cartilage of the ray cartilage, soak it in water for 2 hours, put it in a cooking pot and cook it at 100°C for 30 minutes, remove the fish meat and oil, wash it with water, chop it into pieces with a size of 1cm×1cm, and set aside; For 500g of cartilage, add water according to the ratio of solid to liquid 1:2, adjust the pH=7.0 with sodium hydroxide or hydrochloric acid, add papain 5g / kg, neutral protease 10g / kg and chondroitinase 5g / kg. Control the temperature at 45°C for enzymolysis for 5 and 6 hours, raise the temperature of the obtained enzymolysis solution to 90°C for heat treatment for 13 minutes, then lower the temperature to 70°C, centrifuge for 30 minutes at a speed of 4000 r / min, and add an appropriate amount of filter aid diatom The supernatant obtained by soil filtration was intercepted by an ultrafiltration membrane with a molecular weight of 5000, and the membrane permeate was collected and spray-dried to obtain a cartilage extract.

[0036] The p...

Embodiment 3

[0038] Wash the shark cartilage, soak it in water for 2 hours, put it in a cooking pot and cook it at 100°C for 30 minutes, take it out, remove the fish meat and oil, rinse it with water, chop it into 1cm×1cm pieces, and set aside; choose the processed Cartilage 500g, add water according to the ratio of material to liquid 1:2, adjust pH=7.0 with sodium hydroxide or hydrochloric acid, add papain 5g / kg, neutral protease 5g / kg and chondroitinase 10g / kg. Control the temperature at 45°C for enzymolysis for 5 and 6 hours, raise the temperature of the obtained enzymolysis solution to 100°C for heat treatment for 10 minutes, then lower the temperature to 70°C, use 6000r / min speed, centrifuge for 15 minutes, add an appropriate amount of filter aid kaolin The supernatant obtained by the filtration aid was intercepted by an ultrafiltration membrane with a molecular weight of 5000, and the membrane permeate was collected and spray-dried to obtain a cartilage extract.

[0039] The prepared f...

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PUM

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Abstract

The invention discloses a production method of a fish cartilage extract for preventing and treating osteoarthritis. According to the method, enzymolysis is carried out on fish cartilages by using mixed enzymes; trapping with an ultrafiltration membrane to obtain an extract containing low-molecular-weight chondroitin sulfate of which the molecular weight is less than 1000 Dalton and collagen peptide. In the method, a compound enzyme hydrolysis technology is adopted, so that synchronous hydrolysis of the chondroitin sulfate and collagen is realized, and comprehensive utilization of the fish cartilages is realized. The obtained product is low-molecular-weight chondroitin sulfate, so that the problem of low bioavailability of the chondroitin sulfate on the aspect of clinical application is solved. The extract can remarkably improve the symptoms of osteoarthritis and has good health care and treatment effects.

Description

technical field [0001] The invention belongs to the technical field of production of fish cartilage extracts, and in particular relates to a production method of fish cartilage extracts for preventing and treating osteoarthritis. Background technique [0002] Chondroitin sulfate and collagen are the main biopolymers that form the cartilage matrix. Generally, chondroitin sulfate, as one of the components of cartilage structure, can accelerate the repair of joints, retain water, and quickly penetrate nutrients into cartilage. The type II collagen in cartilage tissue is an important component of cartilage regeneration. The pathophysiology of human osteoarthritis mainly affects the two components of type II collagen and chondroitin sulfate in the cartilage matrix, and is characterized by the breakdown of articular cartilage collagen fibers and the progressive loss of proteoglycans. Therefore, chondroitin sulfate and bone collagen are usually widely used as health food and medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/60A23L1/29A23L1/327A61P19/02A61P19/00A23L17/20A23L33/00
Inventor 刘少芳杨庆利曹荣军余玉奎台文静陈杰姚秀芬鲁涛
Owner QINGDAO BETTER BIO TECH
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