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Bacillus amyloliquefaciens Sh1 microcapsule and preparing method therefor

A technology of amylolytic spores and microcapsules, applied in applications, animal feed, animal feed, etc.

Inactive Publication Date: 2014-03-12
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far no one has used spray drying technology to produce microbial additives for aquaculture feed

Method used

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  • Bacillus amyloliquefaciens Sh1 microcapsule and preparing method therefor
  • Bacillus amyloliquefaciens Sh1 microcapsule and preparing method therefor
  • Bacillus amyloliquefaciens Sh1 microcapsule and preparing method therefor

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Effect test

preparation example Construction

[0047] Preparation of Bacillus amyloliquefaciens Sh1 suspension

[0048] Inoculate Bacillus amyloliquefaciens Sh1 in sterile nutrient broth, culture it on a shaking table at 30°C and 150r / min for 24h, then centrifuge the culture solution at 4°C and 4000r / min for 20min, wash twice with sterile saline Finally, adjust the concentration of the Bacillus amyloliquefaciens Sh1 suspension to 3.6×109cfu / mL with sterile physiological saline, and store it in a refrigerator at 4°C for later use.

Embodiment 1

[0050] Aseptically take 1mL of Bacillus amyloliquefaciens Sh1 suspension and add it to sterile gelatin solutions with concentrations of 2%, 3%, 4%, 5%, and 6%, respectively, and then stir on a magnetic stirrer at 30°C and 100r / min. Mix thoroughly and evenly, and spray dry under the conditions of air inlet temperature 130°C, feed rate 10ml / min, air flow 700L / h, needle pass frequency 10s, etc., respectively for Bacillus amyloliquefaciens Sh1 prepared under different gelatin solution concentrations Microcapsules were used to determine the effective bacterial content.

Embodiment 2

[0052] Aseptically take 1mL of the suspension of Bacillus amyloliquefaciens Sh1 and add it to the sterile gelatin solution with a concentration of 3%, then fully mix evenly on a magnetic stirrer at 30°C and 100r / min, and set the temperature of the air inlet at 110 ℃, 120°C, 130°C, 140°C, 150°C, 160°C, 170°C, and the feed rate is 10ml / min, the air flow rate is 700L / h, and the needle frequency is 10s. The Bacillus amyloliquefaciens Sh1 microcapsules prepared below were used for the determination of the effective bacterial content.

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Abstract

The invention discloses a bacillus amyloliquefaciens Sh1 microcapsule. A microcapsule particle is approximately ball-shaped, depressions exist on the surface of the microcapsule particle, but apertures and cracks do not exist on the surface of the microcapsule particle. The particle size of the microcapsule particle is 8-12 [mu]m, the particles are evenly distributed, and the logarithm value of effective bacterial amount is 6.64-9.28. The invention also provides a preparing method for the microcapsule, and the method comprises following steps: (1) uniformly mixing the bacillus amyloliquefaciens Sh1 solution, emulsion, suspension or pulp with aseptic gelatin solution; and (2) spray drying the mixed product obtained by step (1), and finally preparing microcapsule powder. The method is characterized in that the concentration of the aseptic gelatin utilized in the step (1) is 2-6%.

Description

technical field [0001] The invention belongs to the technical field of microbial additives for aquatic feeding, and in particular relates to a Bacillus amyloliquefaciens Sh1 microcapsule and a preparation method thereof. The Bacillus amyloliquefaciens Sh1 has been disclosed in a Chinese invention patent with the application number 201010264180.6, the applicant is Shanghai Ocean University, and the name is a Saprolegnia antagonist and its application. The corresponding biological deposit information is: CCTCC No: M20101292010.06.02 ). Background technique [0002] In recent years, the rapid development of intensive aquaculture industry has led to the excessive abuse of antibiotics as therapeutic agents and growth promoters for aquatic animal diseases, which has increasingly challenged the safety of aquatic products and caused a series of environmental, health and social problems. At present, developed countries such as Europe, the United States, and Japan have banned antibio...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16
Inventor 曹海鹏邱纬晨吕利群何珊
Owner SHANGHAI OCEAN UNIV
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