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LC/MS (liquid chromatography-mass spectrometer) metabonomics analysis method based on serum of GDM (gestational diabetes mellitus) patient

A technology of gestational diabetes and metabolomics, applied in the field of LC/MS metabolomics analysis based on gestational diabetes human serum

Inactive Publication Date: 2014-02-19
HUZHOU CENT HOSPITAL
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Problems solved by technology

Due to the regional and racial differences in the diagnosis of GDM, there is no unified standard in China at present. Therefore, it is particularly important to find a method for early diagnosis of gestational diabetes in China.

Method used

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  • LC/MS (liquid chromatography-mass spectrometer) metabonomics analysis method based on serum of GDM (gestational diabetes mellitus) patient
  • LC/MS (liquid chromatography-mass spectrometer) metabonomics analysis method based on serum of GDM (gestational diabetes mellitus) patient
  • LC/MS (liquid chromatography-mass spectrometer) metabonomics analysis method based on serum of GDM (gestational diabetes mellitus) patient

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Embodiment Construction

[0024] The present invention is further described below in conjunction with embodiment.

[0025] 1. Data information and analysis content

[0026] 1.1 Basic information of samples

[0027] A total of 60 samples of human serum samples were received from the customer, 2 groups: group A (normal pregnant women) and group B (gestational diabetes pregnant women, the sample numbers are shown in Table 1. According to the contract, LC-MS was performed on each group of samples Positive ion mode analysis and metabolomics analysis.

[0028] Table 1: Human serum samples

[0029] Time (min) Flow rate (mL / min) A (%) B (%) 0 0.4 95 5 2 0.4 95 5 17 0.4 5 95 19 0.4 5 95

[0030] 1.2 Sample preparation

[0031] Take frozen samples, thaw at room temperature for 15 minutes, and vortex for 5 seconds. Take 100 μL of samples and add 300 μL of HPLC grade methanol, vortex for 30 seconds, and let stand at 4°C for 20 minutes; all samples a...

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Abstract

The invention discloses an LC / MS (liquid chromatography-mass spectrometer) metabonomics analysis method based on serum of a GDM (gestational diabetes mellitus) patient. The method comprises the steps as follows: serum samples of a normal pregnant woman and a pregnant woman with GDM are collected, processed and separated by a chromatographic column, and mass spectrometric detection analysis is adopted; original data of an instrument is extracted and aligned after LC / MS detection analysis, and data free of noise disturbance and capable of being used for statistical analysis is obtained; and a multi-dimensional statistical model is established and visually displays metabolic profiling difference between the two groups, so that differential metabolites of the two groups are obtained, and the substances are subjected to preliminary evaluation. With the adoption of the method, the variation condition of the metabolites of the GDM patient can be presented comprehensively and synthetically, and the method can be used for providing powerful technical support for early diagnosis and prognosis of GDM.

Description

technical field [0001] The invention relates to a metabolomics analysis method based on human serum of gestational diabetes LC / MS, in particular to a method for analyzing metabolites in serum of normal pregnant women and pregnant women with gestational diabetes using LC / MS positive ion mode analysis. Background technique [0002] Diabetes is a disease that seriously endangers human life and health. With the continuous improvement of human living standards, accelerated pace of life and unscientific diet, the incidence of gestational diabetes is increasing year by year. Gestational diabetes mellitus (GDM) is a special type of diabetes, which refers to the absence of diabetes and impaired glucose tolerance before pregnancy, diabetes or impaired glucose tolerance that occurs or is first discovered during pregnancy, excluding patients with known diabetes before pregnancy. Prevalence varies from 1% to 14%, depending on the population studied and the diagnostic test criteria used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 钟婧郑淑莺戴利成
Owner HUZHOU CENT HOSPITAL
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