Salt-resistant denitriding composite inoculant, and preparation method and use method thereof

Abstract

The invention belongs to the biotechnical field, and concretely relates to a salt-resistant denitriding composite inoculant, and a preparation method and a use method thereof. The salt-resistant denitriding composite inoculant is obtained by mixing Halomonas having a denitrification characteristic and having nitrite accumulation in the denitriding process, Halomonas having a denitrification characteristic and having no nitrite accumulation in the denitriding process, bacillus having a denitrification characteristic, and Halomonas having a salt resisting characteristic according to a volume ratio of 1:1:(10-20):(60-85); and by adopting the salt-resistant denitriding composite inoculant to denitride high salinity wastewater, the denitriding rate is not lower than 95%, and the treated water is discharged in a standard reaching manner. Four microbes in the composite denitriding interact with each other and influence each other, the removal rate of ammonia nitrogen and total nitrogenof the inoculant is higher than that of the individual use of the microbes; and the impact tolerance of the composite inoculant is stronger than that of a purified strain, so the synchronous implementation of nitration and denitrification is effectively promoted, and the denitriding effect of the high salinity wastewater is obviously improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a salt-tolerant and denitrification compound bacterial agent and its preparation and application method. Background technique [0002] In addition to a large amount of organic pollutants, high-salt wastewater also contains a large amount of chloride ions, which has a negative impact on the traditional biochemical treatment of high-salt wastewater. Inorganic salts play an important role in promoting enzyme reactions, maintaining membrane balance and regulating osmotic pressure during the growth of microorganisms. However, if the concentration of chloride ions is too high, it will inhibit the growth of microorganisms. The main reasons are: [0003] (1) When the salt concentration is too high, the osmotic pressure is high, which dehydrates the microbial cells and causes cell protoplasm separation; [0004] (2) In the case of high salt concentration, the salting-out effect ...

AI Technical Summary

Problems solved by technology

[0004] (2) In the case of high salt concentration, the salting-out effect will reduce the activity of dehydrogenase;

[0005] (3) High chloride ion concentration is toxic to bacteria;

[0006] (4) Due to the increase of water density, activated sludge is easy to float and lose

At present, the treatment of high-salt wastewater can be divided into two categories: physical chemical method and biological method. Due to the high content of dissolved organic matter in this type of wastewater, general physical and chemical methods are difficult to deal with and the treatment cost is high. Biological treatment method is economical, Efficient and harmless, it is still the preferred treatment method

However, when the wastewater contains temporary toxic substances, which are toxic to microorganisms and treated with general biological methods, the degradation rate is slow, and the bacteria need a long period of time to adapt, and the target pollution can be quickly and effectively degraded by adding dominant strains thing

Since the biological treatment system is a very complex system, the best treatment state cannot be achieved by adding a single strain