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Method for culturing oil-producing microalgae by using brewery sewage

A technology for oil-producing microalgae and breweries, applied in the direction of microorganism-based methods, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problem of ineffective use of sewage and achieve low cost, high social and economic benefits , Improve the effect of algae concentration

Active Publication Date: 2014-02-19
ENERGY & ENVIRONMENT RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing brewery sewage cannot be effectively utilized and generate benefits, and to provide a method for cultivating oil-producing microalgae using brewery sewage

Method used

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Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0013] Specific embodiment one: the present embodiment utilizes brewery sewage to cultivate the method for oil-producing microalgae, realizes according to the following steps:

[0014] 1. Inoculate oleaginous microalgae into BG medium, the inoculum amount is 20% (v / v), and cultivate at 25-35°C to a concentration of 2.0×10 7 ~1.0×10 8 cfu / ml;

[0015] 2. Add the brewery sewage treated by the membrane bioreactor (MBR) to the BG medium. The concentration of the brewery sewage in the BG medium is 40% to 60%, and then the concentration in the inoculation step 1 is 2.0×10 7 ~1.0×10 8 cfu / ml oil-producing microalgae, the inoculum amount is 20%-50% (v / v), and placed in a light incubator at a temperature of 25-35°C, a pH of 7-10, and a photoperiod of 10-10 16h, the light intensity is 2000-5000lux, and the ventilation volume is 0.01-0.2VVm, and the light culture is 48-144h;

[0016] 3. Inoculate the oleaginous microalgae after light cultivation into the BG medium containing 40% to 6...

specific Embodiment approach 2

[0024] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, oleaginous microalgae are inoculated into BG medium, the inoculum amount is 20% (v / v), and they are cultivated at 30°C to a concentration of 5.0×10 7 cfu / ml. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0025] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that in step two, the brewery sewage treated by the membrane bioreactor is added to the BG medium, and the concentration of the brewery sewage in the BG medium 50%. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides a method for culturing oil-producing microalgae by using brewery sewage, which relates to a culture method for microalgae. The objective of the invention is to overcome the problems that brewery sewage cannot be effectively utilized and produce benefits in the prior art. The method provided by the invention comprises the following steps: 1, enlarged cultivation; 2, addition of brewery sewage into a culture medium and illumination culture; 3, dark culture; and 4, filtration with a filter membrane and collection. With the method for culturing oil-producing microalgae by using the brewery sewage, substances in the brewery sewage are reused, the concentration of microalgae and the contents of oil and protein are increased, the dual effects of environmental protection and reutilization of biological production resources are achieved, and the advantages of simple and fast operation, low cost, no pollution and high social and economic benefits are obtained. Microalgae consume inorganic substances in the sewage through illumination culture and consume organic substances through dark culture, accumulation of oil is realized, so the problems of sewage pollution and poor oil accumulation in growth of microalgae are overcome and high efficiency oil production of oil-producing microalgae is realized.

Description

technical field [0001] The invention relates to a method for cultivating microalgae. Background technique [0002] Energy shortage and environmental pollution are problems faced by countries all over the world today. Sewage recycling is an important way to solve environmental pollution, and sewage is rich in organic matter. Microalgae play a very important role in solving energy problems. Microalgae is a kind of microorganism capable of photosynthesis. It is a producer in the ecosystem and plays a huge role in the entire material cycle. It has high production efficiency and attractive development prospects. Microalgae are rich in oils, proteins, carbohydrates, trace elements and other biologically active substances, so they can not only be used as bait, but also one of the main sources of food and bioenergy. [0003] Brewery sewage is rich in nutrients and various elements, and direct discharge will pollute the environment. Using other sewage treatment methods requires mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89
Inventor 王志成张宇张玥
Owner ENERGY & ENVIRONMENT RES INST OF HEILONGJIANG PROVINCE
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