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Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm

A technology of leaf extract, Streptococcus suis, applied in the field of pharmacological use of clove leaf extract

Inactive Publication Date: 2014-02-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the inhibitory or elimination effect of clove leaf on the biofilm of Streptococcus suis

Method used

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  • Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm
  • Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm
  • Application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of streptococcus suis biofilm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of clove leaf extract

[0026] Measure 50g of clove leaves, add 500mL of 40% ethanol after crushing, soak in the greenhouse for one hour, extract at 65°C for 3h, pour out the liquid, filter with 12 layers of gauze, add 40% ethanol, 300mL, extract at 65°C for 60min, pour out the liquid and use the same Filter with 12 layers of gauze, concentrate the combined liquid medicine to a certain concentration, and freeze-dry the filtrate by a freeze dryer. Precisely weigh 1g of the freeze-dried powder, dissolve it with 4mL deionized water, centrifuge to take the supernatant, filter it with a 0.22μm filter membrane, and store it at 4°C for later use.

experiment example 1

[0027] Experimental example 1 Intervention and elimination of clove leaf extract on S. suis

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PUM

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Abstract

The invention discloses an application of clove leaf extractives in inhibition of streptococcus suis and intervention and elimination of a streptococcus suis biofilm. The intervention effect of the clove leaf extractives to the formation of the streptococcus suis biofilm and the elimination effect of the clove leaf extractives to the mature streptococcus suis biofilm are researched by using a crystal violet method and a scanning electron microscope. An experiment result proves that the clove leaf extractives have a remarkable intervention effect on the formation of the streptococcus suis biofilm and a remarkable elimination effect for the mature streptococcus suis biofilm, so that the clove leaf extractives can be used for inhibiting the adhesion of the streptococcus suis and the formation of the streptococcus suis biofilm and can take the elimination effect on the mature biofilm. The invention provides a novel treating approach for relevant infections caused by the streptococcus suis biofilm.

Description

Technical field [0001] The present invention relates to a new pharmacological use of clove leaf extract, in particular to the use of clove leaf extract in inhibiting Streptococcus suis, intervening in the formation of Streptococcus suis biofilm, and eliminating the mature biofilm of Streptococcus suis, belonging to the pharmacology of clove leaf extract Field of use. Background technique [0002] Streptococcus suis (S.suis) is an important zoonotic pathogen, which can infect pigs and cause meningitis, pneumonia, arthritis, sepsis, etc. It has strong pathogenicity, high fatality rate and drug resistance Strong, it is an important pathogen causing pig disease. In recent years, due to the widespread use of broad-spectrum antibiotics, the sensitivity of S. suis to almost all antibiotics has been declining, causing the infection rate of S. suis to increase year by year. The resistance mechanism of S.suis includes many aspects, one of which is that it can form a layer of biofilm (BF)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61P31/04A61K127/00
Inventor 李艳华韩强陈俭清刘冰赵玉林盛尊来黄全勇周永辉
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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