Rapid detection kit for aeromonas schubertii and detection method
A detection kit, Aeromonas technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of low accuracy, time-consuming, etc., achieve moderate size, improve specificity, and reduce detection. effect of time
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Embodiment 1
[0017] Rapid detection kit for Aeromonas schubert, and use of the kit for specific detection of genomic DNA of different bacteria.
[0018] The kit consists of:
[0019] The composition of the DNA extraction reagent is 5mM Tris, 0.5mM EDTA pH 8.0 TE buffer; proteinase K, concentration 15mg / mL; 100% chloroform; 100% isopropanol; ethanol, concentration 70%; double distilled water ;
[0020] The PCR reaction reagents were divided into several reaction tubes, and each PCR reaction tube contained 2.5 μL of 10×PCR reaction buffer, 0.5 μL of dNTPs at a concentration of 10 μM, and 0.3 μL of Taq DNA polymerase at a concentration of 5 U / μL at a concentration of 10 μM 0.5 μL of specific oligonucleotide primer A, 0.5 μL of 10 μM specific oligonucleotide primer B, 19.2 μL of sterilized deionized water.
[0021] The detection method of the test kit provided by the present invention to Aeromonas schubert in the sample is to first use the DNA extraction reagent to carry out DNA extraction f...
Embodiment 2
[0033] Aeromonas Schubert rapid detection kit, and the specific detection of genomic DNA of different bacteria using the kit.
[0034] The kit consists of:
[0035] The DNA extraction reagent consists of 15mM Tris, 1.5mM EDTA, pH 8.0 TE buffer; proteinase K, concentration 18mg / mL; 100% chloroform; 100% isopropanol; ethanol, concentration 70%; double distilled water ;
[0036] The PCR reaction reagents are distributed in several reaction tubes, each PCR reaction tube contains 2.5 μL of 10× PCR reaction buffer, 0.5 μL of dNTPs at a concentration of 10 μM, and 0.3 μL of Taq DNA polymerase at a concentration of 5 U / μL at a concentration of 10 μM. 0.5 μL of specific oligonucleotide primer A, 0.5 μL of 10 μM specific oligonucleotide primer B, and 19.2 μL of sterilized deionized water.
[0037] The method for detecting Aeromonas Schubert in a sample by the kit provided by the present invention is to first extract DNA from the sample with a DNA extraction reagent, and then use a PCR...
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