Method of producing low-temperature lipase by fermentation

Abstract

The invention provides a method of producing low-temperature lipase by fermentation. The low-temperature lipase is prepared by using a self-screened wild bacterial strain as starting culture and adopting an optimized seed culture medium, an enzyme-production culture medium as well as a fermentation production technical method. Fermentation liquor has highest enzyme activity of over 6000 U / L; the produced low-temperature lipase has an optimal temperature of 30 DEG C, still has 65% of relative enzyme activity at 0 DEG C, and can be widely applied to the fields such as detergents, food industry, papermaking, medicines, leather and environmental protection, which belong to the biological technical field.

Description

technical field [0001] A method for fermenting and producing low-temperature lipase provided by the invention is to prepare low-temperature lipase by using the wild bacterial strain screened by oneself as the starting strain, and adopting optimized seed culture medium, enzyme-producing medium and fermentation production technology method to prepare low-temperature lipase. The highest enzyme activity of the prepared low-temperature lipase can reach more than 6000U / L, the optimal temperature of the produced low-temperature lipase is 30°C, and it still has 65% relative enzyme activity at 0°C, which can be widely used in detergent, food industry, papermaking , medicine, leather, environmental restoration and other fields, which belong to the field of biotechnology. Background technique [0002] Lipase (lipase, EC3.1.1.3), also known as triacylglycerol acylhyrolase, can catalyze the hydrolysis of its water-insoluble natural substrate triglyceride into fatty acids and glycerol at ...

AI Technical Summary

Problems solved by technology

[0003] Lipase can be divided into acidic, neutral, and alkaline lipases according to their action pH; and can be divided into low-temperature lipase (cold-active lipase), medium-temperature lipase and high-temperature lipase according to their action temperature , Low-temperature lipase is mostly produced by low-temperature microorganisms. The growth conditions of such bacteria are relatively harsh, which seriously restricts the development of low-temperature lipase, so the development and research time is relatively short. However, low-temperature lipase has high catalytic activity in low-temperature environments. Its activation energy and reaction optimum temperature are low, which is beneficial to save energy and prevent product structure damage. It is also of great significance to environmental protection and restoration, because most of the ambient temperature is below 40°C, and low-temperature lipase is used in some fields With the incomparable superiority of medium and high temperature lipase, the research and development and production and preparation technology of low temperature lipase have begun to be valued by countries all over the world. Under the guidance of this situation and ideology, we have developed an effective preparation of low temperature lipase. method