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Graphene capacitive biosensor and methods for manufacturing and detecting same

A technology of biosensors and detection methods, applied in the field of analytical chemistry, can solve the problems that biosensors have not yet been reported, etc.

Inactive Publication Date: 2014-02-05
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Graphene biosensors based on capacitive effects have not been reported yet

Method used

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  • Graphene capacitive biosensor and methods for manufacturing and detecting same
  • Graphene capacitive biosensor and methods for manufacturing and detecting same
  • Graphene capacitive biosensor and methods for manufacturing and detecting same

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Embodiment Construction

[0041] The present invention is described in detail below in conjunction with accompanying drawing:

[0042] Such as figure 1 As shown, a graphene capacitive biosensor comprises a glass substrate 1, both sides of the glass substrate 1 are provided with ITO2, part of the glass substrate 1 and ITO2 on the same side are covered with graphene 3, and there is no graphene 3. The glass substrate 1 and the ITO2 on both sides are located on the same side, and the ITO2 on both sides not covered with the graphene 3 is a pole 7 of the capacitor. The top of the ITO2 covered with the graphene 3 is covered with a PET gasket 4, and the PET gasket 4 The PET substrate 5 covered with the sputtered gold film 6 is provided with a sample cell in the middle of the glass substrate 1 by using insulating silica gel, and the gold film 6 is the other pole 8 of the capacitor.

[0043] The size of the glass substrate 1 not covered with graphene 3 and the ITO2 on both sides are 20mm*20mm and 20mm*5mm resp...

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Abstract

The invention discloses a graphene capacitive biosensor and methods for manufacturing and detecting the same. The graphene capacitive biosensor comprises a glass substrate, wherein indium tin oxides (ITO) are arranged on the two sides of the glass substrate respectively; graphene covers part of the glass substrate on the same side and the ITOs; the glass substrate which is not covered with the graphene and the ITOs on the two sides are positioned on the same side; each of the ITOs on the two sides of the glass substrate which is not covered with the graphene is an electrode of a capacitor; polyethylene terephthalate (PET) gaskets cover the ITOs which are covered with the graphene; a PET substrate to which a gold film is sputtered covers each PET gasket; a sample cell is formed in the middle of the glass substrate by insulating silicon gel; the gold film is the other electrode of the capacitor. By using a method for covering the electrodes of the ITOs with the graphene, the contact resistance is reduced, instantaneous voltage pulse is applied, and the capacitor responds for measurement. Adenosine triphosphate is taken as an experiment object, and the detection lower limit of the adenosine triphosphate reaches 1pM through the method.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a graphene capacitive biosensor, a manufacturing method and a detection method thereof. Background technique [0002] Graphene is an emerging functional material. Due to its excellent optical, electrical and easy to combine with biomolecules through π-π stacking, it has attracted attention in the field of biosensor design. Adsorption of biomolecules on the graphene surface can regulate its charge transport or channel doping of charges, causing changes in carrier concentration and carrier mobility. [0003] Graphene supercapacitor is a general term for supercapacitors based on graphene materials. Due to graphene's unique two-dimensional structure and excellent intrinsic physical properties, such as exceptionally high electrical conductivity and large surface area, graphene-based materials have great potential for applications in supercapacitors. Compared with traditional ele...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/22
Inventor 岳伟伟许士才姜守振白成杰
Owner SHANDONG NORMAL UNIV
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