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Method for identifying heat resistance of capsicum by utilization of in vitro leaf disk

A technology of detached leaves and heat resistance, applied in the fields of weather resistance/light resistance/corrosion resistance, measuring devices, instruments, etc., can solve the problems of long time required, difficulty in mass production, poor reproducibility of results, etc., and achieve identification results Consistent, improved process, reproducible results

Inactive Publication Date: 2014-02-05
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Field identification is greatly affected by environmental conditions, the reproducibility of the results is poor, the time required is long, and the workload is heavy;
[0005] Artificial simulated adversity identification method requires certain facility conditions and is difficult to carry out in large quantities;
[0006] Physiological identification of adversity is an indirect identification method, and its reliability is poor
[0007] The above-mentioned existing identification methods are time-consuming, laborious and inefficient, especially for some strains and transgenic strains with few samples available for identification, and have great limitations.

Method used

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  • Method for identifying heat resistance of capsicum by utilization of in vitro leaf disk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In this example, the detached leaf discs of pepper 6-7 leaves at the seedling stage are used to carry out heat resistance identification in an artificial climate box, and the heat damage index of a certain strain group to be tested is calculated by statistics, and according to the created group heat damage index The grading standard can identify the heat resistance of peppers. Specific steps are as follows:

[0037] 1. The heat-resistant pepper strain B35 was used as the heat-resistant control, and the heat-labile pepper strain B6 was used as the heat-sensitive control (Zhu Wei, Lu Minghui, Gong Zhenhui, and Chen Rugang. Cloning and Expression under Heat Stress of A Small Heat Shock Protein Gene CaHSP24 from Pepper[J].African Journal of Biotechnology, 2011, 10(25):4968-4976),

[0038] The pepper strains to be tested were PP97-7195-1 and Mito Lee Seln-1 (both pepper strains well-known in the art, provided by the applicant's research group).

[0039]2. Soak the seeds of...

Embodiment 2

[0059] In this example, the detached leaf discs of pepper 6-7 leaves at the seedling stage are used to carry out heat resistance identification in an artificial climate box, and the heat damage index of a certain strain group to be tested is calculated by statistics, and according to the created group heat damage index The grading standard can identify the heat resistance of peppers. Specific steps are as follows:

[0060] 1. The heat-resistant pepper strain B35 was used as the heat-resistant control, and the heat-labile pepper strain B6 was used as the heat-sensitive control (Zhu Wei, Lu Minghui, Gong Zhenhui and Chen Rugang. Cloning and Expression under Heat Stress of A Small Heat Shock Protein Gene CaHSP24 from Pepper[J].African Journal of Biotechnology, 2011, 10(25):4968-4976),

[0061] The pepper strains to be tested were Huangyuanshuai No. 2 selection, Meiyu selection, Baozijiao and EarlyCalwonder (all pepper strains well-known in the art, provided by the applicant's re...

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Abstract

The invention discloses a method for identifying heat resistance of capsicum by the utilization of in vitro leaf disk. The method mainly includes capsicum heatproof line B35 used as a heatproof contrast, capsicum heat-labile line B6 used as a temperature-sensitive contrast, heat resistance identification of capsicum in vitro leaf disk, heat damage grading standard of in vitro leaf disk and the like. The method is characterized in that when the capsicum in vitro leaf disk is processed at specific temperature (high temperature and normal temperature), at specific concentration of a salt solution and within a certain period of time, different heatproof lines have distinct differences in leaf disk phenotype symptom. On this account, through statistics and calculation of heat damage index of some line and according to the established heat damage grading standard, heat resistance lines and heat sensitivity lines of capsicum can be identified. The method is especially suitable for identification and genetic analysis of heat resistance of those rare and transgenic lines of capsicum material, is a method which is simple and rapid to operate and effectively identifies heat resistance of the capsicum germplasm resources, and can be adopted to accelerate the progress of capsicum heatproof breeding.

Description

technical field [0001] The invention belongs to the field of vegetable breeding, and in particular relates to a method for distinguishing different heat-resistant pepper germplasm resources by using isolated pepper leaf discs, in particular to heat resistance identification of isolated pepper leaf discs, and isolated pepper leaf discs. Method for establishment of sheet heat resistance grading system. This method is used to identify heat-resistant plants on the premise of ensuring the survival of heat-resistant plants, which is convenient for saving heat-resistant strains and genetic analysis of offspring, and is especially suitable for rare pepper breeding lines such as small strain populations and transgenic strains. It is a simple, fast and effective method to identify the heat tolerance of a large number of pepper germplasm resources. Background technique [0002] Pepper (Capsicum annuum L.) is a typical thermophilic plant, and high temperature is not conducive to its gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N17/00
Inventor 巩振辉田士林郭卫丽
Owner NORTHWEST A & F UNIV
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