A method for identifying the heat tolerance of pepper by using isolated leaf discs

Abstract

The invention discloses a method for identifying the heat resistance of capsicum by using isolated leaf discs. The method mainly includes taking the heat-resistant capsicum strain B35 as the heat-resistant control, using the capsicum heat-intolerant strain B6 as the heat-sensitive control, and the capsicum in vitro Heat resistance identification of leaf discs, heat damage classification standards for isolated leaf discs, etc. The characteristic of this method is that the isolated leaf discs of pepper are treated at specific temperature (high temperature and normal temperature), specific concentration of salt solution and for a certain period of time, and there are obvious differences in leaf disc phenotype symptoms among different heat-resistant strains. Through statistics and calculation of the heat damage index of a certain strain group to be tested, and according to the established group heat damage classification standard, heat-resistant and heat-sensitive pepper strains can be identified. This method is especially suitable for the identification and genetic analysis of the heat resistance of those rare and transgenic pepper materials. It is a simple, fast and effective method for identifying the heat resistance of pepper germplasm resources, which can greatly accelerate the heat resistance Hot breeding process.

Description

technical field [0001] The invention belongs to the field of vegetable breeding, and in particular relates to a method for distinguishing different heat-resistant pepper germplasm resources by using isolated pepper leaf discs, in particular to heat resistance identification of isolated pepper leaf discs, and isolated pepper leaf discs. Method for establishment of sheet heat resistance grading system. This method is used to identify heat-resistant plants on the premise of ensuring the survival of heat-resistant plants, which is convenient for saving heat-resistant strains and genetic analysis of offspring, and is especially suitable for rare pepper breeding lines such as small strain populations and transgenic strains. It is a simple, fast and effective method to identify the heat tolerance of a large number of pepper germplasm resources. Background technique [0002] Pepper (Capsicum annuum L.) is a typical thermophilic plant, and high temperature is not conducive to its gr...

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Problems solved by technology

[0004] Field identification is greatly affected by environmental conditions, the reproducibility of the results is poor, the time required is long, and the workload is heavy;

[0005] Artificial simulated adversity identification method requires certain facility conditions and is difficult to carry out in large quantities;

[0006] Physiological identification of adversity is an indirect identification method, and its reliability is poor

[0007] The above-mentioned existing identification methods are time-consuming, laborious and inefficient, especially for some strains and transgenic strains with few samples available for identification, and have great limitations.

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