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Mycobacterium tuberculosis sputum specimen liquefier kit and application thereof

A technology of Mycobacterium tuberculosis and liquefaction agent, which is applied in the field of medical specimen detection, can solve problems such as the inability to prepare sputum specimens, achieve the effects of shortening the microscope reading time, improving the quality of smears, and reducing sputum streaks

Inactive Publication Date: 2014-02-05
SHANGHAI WEIYIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is that the current liquefaction agent for tuberculosis sputum specimens cannot prepare sputum specimens that can be used for high-quality smears without centrifugation. In order to overcome the above shortcomings, a liquefaction agent for tuberculosis sputum specimens is provided Kits and their applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Smear after liquefaction of caseous and bloody sputum specimens and microscopic examination of Zel-Nelson's acid-fast staining

[0033] Sputum specimens: 200 caseous and bloody sputum tuberculosis positive specimens, of which 100 were weakly positive specimens below 1+ (including 1+).

[0034] Mycobacterium tuberculosis sputum specimen liquefaction agent kit includes liquefaction agent R1 and liquefaction agent R2: liquefaction agent R1 is ammonia water prepared by 90mL concentrated ammonia water (ammonia mass percentage concentration is 25-28%) and 10mL water, and liquefaction agent R2 is mercapto An aqueous solution of a reducing agent, the thiol reducing agent is composed of β-mercaptoethanol and N-acetyl-L-cysteine, wherein the concentration of β-mercaptoethanol is 30.0mg / mL, N-acetyl-L-cysteine The acid concentration was 40.0 mg / mL.

[0035] Divide the above-mentioned sputum sample into two parts, one part is liquefied and smeared, and the other part is ...

Embodiment 2

[0041] Example 2 Smear and fluorescent acid-fast staining microscopic examination of saliva specimen after liquefaction

[0042] Sputum samples: 180 cases of saliva samples to be tested.

[0043] Mycobacterium tuberculosis sputum specimen liquefaction agent kit includes liquefaction agent R1 and liquefaction agent R2: liquefaction agent R1 is ammonia water prepared by 10mL concentrated ammonia water (ammonia mass percentage concentration is 25-28%) and 90mL water, and liquefaction agent R2 is mercapto An aqueous solution of reducing agent, the mercapto reducing agent is composed of β-mercaptoethanol, N-acetyl-L-cysteine ​​and dithiothreitol, wherein the concentration of β-mercaptoethanol is 3.0mg / mL, N-acetyl - L-cysteine ​​at a concentration of 1.0 mg / mL and dithiothreitol at a concentration of 5.0 mg / mL.

[0044] Divide the above-mentioned sputum sample into two parts, one part is liquefied and smeared, and the other part is smeared directly.

[0045] The process of liquef...

Embodiment 3

[0052] Example 3 Smear and fluorescent acid-fast staining of mixed sputum specimen after liquefaction

[0053] Sputum samples: 150 positive samples of mixed sputum tuberculosis, of which 60 cases were strong positive samples above 1+, and 90 cases were weak positive samples below 1+ (including 1+).

[0054] The mixed sputum sample is a mixture of at least two types of caseous sputum, bloody sputum, mucus sputum and saliva in the sputum sample.

[0055] Mycobacterium tuberculosis sputum specimen liquefaction agent kit includes liquefaction agent R1 and liquefaction agent R2: liquefaction agent R1 is concentrated ammonia water (the mass percent concentration of ammonia is 25-28%), and liquefaction agent R2 is an aqueous solution of sulfhydryl reducing agent. The thiol reducing agent consists of N-acetyl-L-cysteine ​​and dithiothreitol, wherein the concentration of N-acetyl-L-cysteine ​​is 8.0 mg / mL and the concentration of dithiothreitol is 15.0 mg / mL.

[0056] Divide the abov...

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PUM

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Abstract

The invention belongs to the technical field of detection of medical samples, and in particular relates to a mycobacterium tuberculosis sputum specimen liquefier kit and application thereof. The mycobacterium tuberculosis sputum specimen liquefier kit includes a liquefier R1 and a liquefier R2; and the liquefier R1 is ammonia, and the liquefier R2 is a water solution of a sulfydryl reducing agent. The invention also provides application of the above mycobacterium tuberculosis sputum specimen liquefier kit, and the kit can be applied to acid-fast staining microscopic examination of mycobacterium tuberculosis and liquefaction of sputum specimen before isolated culture. Compared with the prior art, the technical scheme provided by the invention has the following advantages: the kit helps to improve smear quality, increases positive detection rate and reading efficiency, optimizes the existing pretreatment technology for sputum specimens, satisfies requirements of multiple detection samples, and is in favor of staining microscopic examination of the sputum specimen smear and automation operation of isolated culture and inoculation.

Description

technical field [0001] The invention belongs to the technical field of medical specimen detection, and in particular relates to a tubercle bacillus sputum specimen liquefier kit and application thereof. Background technique [0002] The detection requirement of conventional tuberculosis sputum specimens is that a sputum specimen should be subjected to both direct smear acid-fast staining microscope examination and isolation culture. The smear staining microscope examination can report immediately, while the isolation culture that takes a long time to report can use Due to the drug resistance test of the specimen, the positive detection rate of the smear staining microscopic examination is low at present, partly because the distribution of Mycobacterium tuberculosis in the sputum specimen is uneven, and the prior art is mainly to pick pus samples manually , Cheese-like sputum samples are directly smeared and then stained for microscopic examination. Therefore, whether the spu...

Claims

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Application Information

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IPC IPC(8): C12Q1/02G01N1/38G01N1/28C12R1/32
Inventor 贺坚慧
Owner SHANGHAI WEIYIN BIOTECH CO LTD
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