Application of atractylodes macrocephalaon polysaccharide in medicaments or healthcare products for improving kuffer cell immune function
A technology of Atractylodes macrocephala polysaccharide and cellular immunity, applied in the field of traditional Chinese medicine
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Embodiment 1
[0019] Example 1 Homemade Atractylodes Polysaccharides (PAM)
[0020] DEAE-cellulose filler was purchased from Beijing Ruida Henghui Technology Development Co., Ltd.
[0021] Take about 1000g of Atractylodes macrocephala, crush it into coarse particles, soak for 30min, heat and reflux with 95% ethanol to degrease for 2h, extract the filter residue three times with 3 times the amount of water, combine the water extracts, and concentrate. The concentrated solution was repeatedly washed three times with ethanol and acetone, and the precipitate was freeze-dried to obtain the crude polysaccharide of Atractylodes macrocephala. Atractylodes rhizome crude polysaccharides were dissolved in a small amount of distilled water, separated on a DEAE-cellulose column, and eluted with distilled water and 0.1mol / L NaCl solution respectively. Polysaccharides were partially eluted with distilled water. Dialyze the eluent, concentrate the dialysate, add ethanol to a concentration of 75% (V / V), l...
Embodiment 2
[0025] Embodiment 2 mouse experiment
[0026] 1. Instruments and materials
[0027] Experimental Animals Healthy BALB / C mice weighing 20-30 g, both male and female, were provided by the Animal Experiment Center of Xi'an Jiaotong University School of Medicine. In an environment with a constant temperature and a photoperiod of 12h:12h, the animals were fed for 1wk and then used for the experiment. Before the experiment, they were fasted for 24h and allowed to drink water.
[0028] Drugs and treatment Atractylodes macrocephala polysaccharide (PAM, self-made, purity greater than 99.9%), extracted from the dried root of Atractylodes macrocephala (identified as Atractylodes Macrocephala Koidz. by Professor Guo Zengjun, Department of Pharmacognosy, School of Medicine, Xi'an Jiaotong University), soluble in water, the main component For arabinose, mannose, glucose, galactose. Dissolve in distilled water before use, sterilize by autoclaving, and take the supernatant after centrifugat...
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